大鼠成骨细胞培养物。

Rat osteoblast cultures.

作者信息

Orriss Isabel R, Taylor Sarah E B, Arnett Timothy R

机构信息

Department of Cell and Developmental Biology, University College London, London, UK.

出版信息

Methods Mol Biol. 2012;816:31-41. doi: 10.1007/978-1-61779-415-5_3.

DOI:10.1007/978-1-61779-415-5_3

PMID:22130920

Abstract

This chapter describes the isolation, culture and staining of primary osteoblasts from the calvaria and long bones of neonatal rats. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralisation, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. A special focus of this chapter is on the role of β-glycerophosphate in cell-mediated mineralisation in these cultures.

摘要

本章描述了从新生大鼠颅骨和长骨中分离、培养和染色原代成骨细胞的方法。该实验的主要优点在于，它能够直接测量骨基质沉积和矿化情况，同时在特定分化阶段产生大量成骨细胞用于分子和组织学分析。本章特别关注β-甘油磷酸在这些培养物中细胞介导矿化过程中的作用。