原代禽骨细胞的分离

Isolation of primary avian osteocytes.

作者信息

Semeins Cor M, Bakker Astrid D, Klein-Nulend Jenneke

机构信息

Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2012;816:43-53. doi: 10.1007/978-1-61779-415-5_4.

DOI:10.1007/978-1-61779-415-5_4

PMID:22130921

Abstract

Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.

摘要

使用温和的乙二胺四乙酸（EDTA）处理并交替进行胶原酶处理，可以从鸡颅骨中分离出骨细胞。所获得的细胞群体包含成骨细胞和骨细胞。在用骨细胞特异性单克隆抗体MAb OB7.3进行免疫磁珠分离后，可获得纯的骨细胞群体。