Laboratory for Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Belgium.
Electrophoresis. 2012 Jan;33(1):211-27. doi: 10.1002/elps.201100366. Epub 2011 Dec 7.
This review provides systematic coverage of examples in the field of in-capillary electrophorecially mediated microanalysis (EMMA). The recent developments and applications in the time period up to mid 2011 have been described, as well as relevant older papers. The basic principles and modes of in-capillary assays have been demonstrated. An overview is also given of the various injection, separation and detection modes implemented in combination with EMMA. The review is presented in two parts mainly dealing with (i) enzymatic and (ii) derivatization or chemical reactions. Finally, the future trends of CE in performing and monitoring reactions have been drawn.
本文综述了毛细管电泳介导的微分析（EMMA）领域的实例。描述了截至 2011 年年中该领域的最新发展和应用，以及相关的较早期文献。文中展示了毛细管内分析的基本原理和模式，并概述了与 EMMA 相结合实施的各种进样、分离和检测模式。本文分为两部分，主要涉及（i）酶和（ii）衍生化或化学反应。最后，探讨了 CE 在进行和监测反应方面的未来趋势。
