安第斯山病毒 NSs 蛋白通过一个渗漏扫描机制从病毒的小 mRNA 上表达。
The Andes hantavirus NSs protein is expressed from the viral small mRNA by a leaky scanning mechanism.
机构信息
Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, Centro de Investigaciones Médicas, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
出版信息
J Virol. 2012 Feb;86(4):2176-87. doi: 10.1128/JVI.06223-11. Epub 2011 Dec 7.
Abstract
The small mRNA (SmRNA) of all Bunyaviridae encodes the nucleocapsid (N) protein. In 4 out of 5 genera in the Bunyaviridae, the smRNA encodes an additional nonstructural protein denominated NSs. In this study, we show that Andes hantavirus (ANDV) SmRNA encodes an NSs protein. Data show that the NSs protein is expressed in the context of an ANDV infection. Additionally, our results suggest that translation initiation from the NSs initiation codon is mediated by ribosomal subunits that have bypassed the upstream N protein initiation codon through a leaky scanning mechanism.
摘要
所有布尼亚病毒科的小 mRNA（SmRNA）编码核衣壳（N）蛋白。在布尼亚病毒科的 5 个属中的 4 个属中，SmRNA 编码另一种非结构蛋白，称为 NSs。在这项研究中，我们表明安第斯山出血热病毒（ANDV）SmRNA 编码 NSs 蛋白。数据表明，NSs 蛋白在 ANDV 感染的情况下表达。此外，我们的结果表明，从 NSs 起始密码子开始的翻译起始是通过核糖体亚基介导的，这些核糖体亚基通过渗漏扫描机制绕过了上游的 N 蛋白起始密码子。
相似文献
1
The Andes hantavirus NSs protein is expressed from the viral small mRNA by a leaky scanning mechanism.
J Virol. 2012 Feb;86(4):2176-87. doi: 10.1128/JVI.06223-11. Epub 2011 Dec 7.
2
The Andes Orthohantavirus NSs Protein Antagonizes the Type I Interferon Response by Inhibiting MAVS Signaling.
J Virol. 2020 Jun 16;94(13). doi: 10.1128/JVI.00454-20.
3
Tula hantavirus NSs protein accumulates in the perinuclear area in infected and transfected cells.
Arch Virol. 2010;155(1):117-21. doi: 10.1007/s00705-009-0546-y. Epub 2009 Dec 3.
4
The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
PLoS Pathog. 2021 Sep 21;17(9):e1009931. doi: 10.1371/journal.ppat.1009931. eCollection 2021 Sep.
5
The 3' untranslated region of the Andes hantavirus small mRNA functionally replaces the poly(A) tail and stimulates cap-dependent translation initiation from the viral mRNA.
J Virol. 2010 Oct;84(19):10420-4. doi: 10.1128/JVI.01270-10. Epub 2010 Jul 21.
6
Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter.
J Med Virol. 2007 Oct;79(10):1527-36. doi: 10.1002/jmv.20948.
7
Tula hantavirus isolate with the full-length ORF for nonstructural protein NSs survives for more consequent passages in interferon-competent cells than the isolate having truncated NSs ORF.
Virol J. 2008 Jan 11;5:3. doi: 10.1186/1743-422X-5-3.
8
Signatures of host mRNA 5' terminus for efficient hantavirus cap snatching.
J Virol. 2012 Sep;86(18):10173-85. doi: 10.1128/JVI.05560-11. Epub 2012 Jul 11.
9
Characterization of the Interaction between hantavirus nucleocapsid protein (N) and ribosomal protein S19 (RPS19).
J Biol Chem. 2011 Apr 1;286(13):11814-24. doi: 10.1074/jbc.M110.210179. Epub 2011 Feb 4.
10
Searching for cellular partners of hantaviral nonstructural protein NSs: Y2H screening of mouse cDNA library and analysis of cellular interactome.
PLoS One. 2012;7(4):e34307. doi: 10.1371/journal.pone.0034307. Epub 2012 Apr 10.
引用本文的文献
1
Orthohantaviruses: An Overview of the Current Status of Diagnostics and Surveillance.
Viruses. 2025 Apr 26;17(5):622. doi: 10.3390/v17050622.
2
Heterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAs.
Nucleic Acids Res. 2024 Mar 21;52(5):2625-2647. doi: 10.1093/nar/gkad1221.
3
The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation.
Nucleic Acids Res. 2022 Jan 11;50(1):411-429. doi: 10.1093/nar/gkab1188.
4
The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.
PLoS Pathog. 2021 Sep 21;17(9):e1009931. doi: 10.1371/journal.ppat.1009931. eCollection 2021 Sep.
5
Hantavirus Replication Cycle-An Updated Structural Virology Perspective.
Viruses. 2021 Aug 6;13(8):1561. doi: 10.3390/v13081561.
6
Inhibition of interferon I induction by non-structural protein NSs of Puumala virus and other vole-associated orthohantaviruses: phenotypic plasticity of the protein and potential functional domains.
Arch Virol. 2021 Nov;166(11):2999-3012. doi: 10.1007/s00705-021-05159-y. Epub 2021 Aug 13.
7
Unconventional viral gene expression mechanisms as therapeutic targets.
Nature. 2021 May;593(7859):362-371. doi: 10.1038/s41586-021-03511-5. Epub 2021 May 19.
8
Chloroquine, an Anti-Malaria Drug as Effective Prevention for Hantavirus Infections.
Front Cell Infect Microbiol. 2021 Mar 15;11:580532. doi: 10.3389/fcimb.2021.580532. eCollection 2021.
9
A Look into Genomes: Functions of Non-Structural (NS) Proteins.
Viruses. 2021 Feb 18;13(2):314. doi: 10.3390/v13020314.
10
Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling.
Viruses. 2021 Jan 19;13(1):140. doi: 10.3390/v13010140.
本文引用的文献
1
Infection of human monocyte-derived dendritic cells by ANDES Hantavirus enhances pro-inflammatory state, the secretion of active MMP-9 and indirectly enhances endothelial permeability.
Virol J. 2011 May 13;8:223. doi: 10.1186/1743-422X-8-223.
2
The 3' untranslated region of the Andes hantavirus small mRNA functionally replaces the poly(A) tail and stimulates cap-dependent translation initiation from the viral mRNA.
J Virol. 2010 Oct;84(19):10420-4. doi: 10.1128/JVI.01270-10. Epub 2010 Jul 21.
3
Tula hantavirus NSs protein accumulates in the perinuclear area in infected and transfected cells.
Arch Virol. 2010;155(1):117-21. doi: 10.1007/s00705-009-0546-y. Epub 2009 Dec 3.
4
The 5'-untranslated region of the mouse mammary tumor virus mRNA exhibits cap-independent translation initiation.
Nucleic Acids Res. 2010 Jan;38(2):618-32. doi: 10.1093/nar/gkp890. Epub 2009 Nov 4.
5
Translation of intronless RNAs is strongly stimulated by the Epstein-Barr virus mRNA export factor EB2.
Nucleic Acids Res. 2009 Aug;37(15):4932-43. doi: 10.1093/nar/gkp497. Epub 2009 Jun 15.
6
Viruses in the Anopheles A, Anopheles B, and Tete serogroups in the Orthobunyavirus genus (family Bunyaviridae) do not encode an NSs protein.
J Virol. 2009 Aug;83(15):7612-8. doi: 10.1128/JVI.02080-08. Epub 2009 May 13.
7
Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein.
Virology. 2009 Jun 5;388(2):279-85. doi: 10.1016/j.virol.2009.03.032. Epub 2009 Apr 25.
8
Translational control of the subgenomic RNAs of severe acute respiratory syndrome coronavirus.
Virus Genes. 2009 Aug;39(1):10-8. doi: 10.1007/s11262-009-0357-y. Epub 2009 Apr 12.
9
Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome.
J Virol. 2009 May;83(10):5046-55. doi: 10.1128/JVI.02409-08. Epub 2009 Mar 11.
10
Analysis of natural variants of the hepatitis C virus internal ribosome entry site reveals that primary sequence plays a key role in cap-independent translation.
Nucleic Acids Res. 2009 Feb;37(3):957-71. doi: 10.1093/nar/gkn1022. Epub 2008 Dec 23.
Zotero 插件