Luminex xTAG 真菌检测法检测血培养中的酵母菌。
Detection of yeasts in blood cultures by the Luminex xTAG fungal assay.
机构信息
Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio, USA.
出版信息
J Clin Microbiol. 2012 Feb;50(2):492-4. doi: 10.1128/JCM.06375-11. Epub 2011 Dec 14.
Abstract
A multiplex-PCR Luminex xMAP bead probe fluid array using xTAG analyte-specific reagents (multiplex xTAG fungal ASR assay) was employed for detection of clinically significant Candida species, Cryptococcus neoformans, Histoplasma capsulatum, and Blastomyces dermatitidis from blood cultures. We tested 132 blood cultures negative (n = 10) or positive (n = 97) for yeasts and/or bacteria (n = 25). The assay showed sensitivity and specificity of 100% and 99%, respectively. The xTAG fungal ASR assay is a rapid assay that allows simultaneous identification of multiple yeast species.
摘要
采用 xTAG 分析物特异性试剂的多重 PCR Luminex xMAP 珠探针液芯片技术（多重 xTAG 真菌 ASR 检测法）用于从血培养物中检测临床重要的念珠菌属种、新型隐球菌、荚膜组织胞浆菌和皮炎芽生菌。我们检测了 132 份血培养物，其中阴性（n = 10）或阳性（n = 97）的酵母菌和/或细菌（n = 25）。该检测法的灵敏度和特异性分别为 100%和 99%。xTAG 真菌 ASR 检测法是一种快速检测法，可同时鉴定多种酵母菌种。
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