Phospholipase A2 activity and concentration in several body fluids in patients with acute pancreatitis.

According to recent studies, phospholipase A2 (PLA2) may be an important factor in the pathogenesis and pathophysiology of acute pancreatitis. Increased serum PLA2 activities and concentrations have been measured in patients with acute pancreatitis. Serum PLA2 activities have been shown to correlate with the severity and prognosis of the disease. To study the different methods of PLA2 determination, we measured the PLA2 activity by means of an isotopic assay method and the concentration by a radioimmunologic method in several body fluids of 52 consecutive patients with acute pancreatitis. PLA2 activity and concentration were detected in all of the patient body fluids. The serum PLA2 activities were 2.5-fold higher (mean +/- SD, 7.6 +/- 6.0) than normal activities, and the concentrations were 9.6-fold higher (mean +/- SD, 41 +/- 88). The enzyme activities and concentrations correlated well in ascites, fluids from the pleural cavity, and peritoneal lavation and poorly in serum, urine, and fluid from pancreatic pseudocyst.