对苏门答腊犀牛（双角犀属苏门答腊种）死后卵母细胞进行体外成熟和受精尝试。

Attempted in vitro maturation and fertilization of postmortem Sumatran rhinoceros (Dicerorhinus sumatrensis) oocytes.

作者信息

Stoops Monica A, Bateman Helen L, Campbell Mark K, Roth Terri L

机构信息

Center for Conservation and Research of Endangered Wildlife, Cincinnati Zoo & Botanical Garden, 3400 Vine Street, Cincinnati, Ohio 45220, USA.

出版信息

J Zoo Wildl Med. 2011 Dec;42(4):723-6. doi: 10.1638/2010-0186.1.

DOI:10.1638/2010-0186.1

PMID:22204070

Abstract

A study was conducted opportunistically to evaluate the potential of rescuing immature oocytes from the ovaries of the Sumatran rhinoceros postmortem. Recovered oocytes (n = 30) were placed in maturation culture for 36 hr and inseminated with frozen-thawed homologous spermatozoa. After culture, evaluation of nuclear maturation status revealed that a large number of oocytes were degenerated (n = 21), but nine oocytes were assessed at the germinal vesicle (n = 3), metaphase I (n = 3), and metaphase II (n = 3) stages. Frozen-thawed Sumatran rhinoceros spermatozoa were capable of binding to the zona pellucida of in vitro matured oocytes, but no fertilization or cleavage resulted. In conclusion, relatively large numbers of oocytes can be obtained by ovarian follicular aspiration postmortem in the Sumatran rhinoceros, and some of these oocytes are capable of achieving nuclear maturation in vitro. However, additional studies are required to improve maturation success and achieve fertilization in culture.

摘要

一项机会性研究旨在评估从苏门答腊犀牛死后卵巢中挽救未成熟卵母细胞的潜力。回收的卵母细胞（n = 30）被置于成熟培养液中培养36小时，并用冻融的同源精子进行授精。培养后，对核成熟状态的评估显示，大量卵母细胞发生退化（n = 21），但有9个卵母细胞分别处于生发泡期（n = 3）、减数分裂中期I（n = 3）和减数分裂中期II（n = 3）阶段。冻融的苏门答腊犀牛精子能够与体外成熟卵母细胞的透明带结合，但未产生受精或卵裂。总之，通过对苏门答腊犀牛进行死后卵巢卵泡抽吸可获得相对大量的卵母细胞，其中一些卵母细胞能够在体外实现核成熟。然而，需要进一步研究以提高成熟成功率并在培养中实现受精。