使用克拉克电极测量线粒体耗氧量。
Measurement of mitochondrial oxygen consumption using a Clark electrode.
作者信息
Li Zhihong, Graham Brett H
机构信息
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
出版信息
Methods Mol Biol. 2012;837:63-72. doi: 10.1007/978-1-61779-504-6_5.
Abstract
Mitochondria require oxygen to produce ATP in sufficient quantities to drive energy-requiring reactions in eukaryotic organisms. The measurement of oxygen consumption rates from isolated mitochondria in vitro is a useful and valuable technique in the research and evaluation of mitochondrial dysfunction and disease since ADP-dependent oxygen consumption directly reflects coupled respiration or oxidative phosphorylation (OXPHOS). This chapter describes the traditional method of mitochondrial polarography using a Clark electrode for measuring coupled respiration in freshly isolated mitochondria from both mammalian tissues and Drosophila melanogaster.
摘要
线粒体需要氧气来产生足够数量的三磷酸腺苷（ATP），以驱动真核生物中需要能量的反应。在体外测量分离线粒体的耗氧率，是研究和评估线粒体功能障碍及疾病的一项有用且有价值的技术，因为依赖二磷酸腺苷（ADP）的耗氧直接反映了偶联呼吸或氧化磷酸化（OXPHOS）。本章描述了使用克拉克电极进行线粒体极谱分析的传统方法，用于测量从哺乳动物组织和黑腹果蝇新鲜分离的线粒体中的偶联呼吸。
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