从海洋海绵 Axinella corrugata 中分离出的 ACL-I 的生物活性和 ACL-II 的理化性质，凝集素。

Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata.

机构信息

Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, 90610-000, Porto Alegre, RS, Brazil.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 2012 Apr;161(4):365-70. doi: 10.1016/j.cbpb.2012.01.001. Epub 2012 Jan 8.

DOI:10.1016/j.cbpb.2012.01.001

PMID:22245532

Abstract

Lectin II from the marine sponge Axinella corrugata (ACL-II) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-II is a lectin with an Mr of 80 kDa and 78 kDa, estimated by SDS-PAGE and by FPLC on Superose 12 HR column, respectively. ACL-II mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of Ca(2+), Mg(2+) and Mn(2+), but is inhibited by d-galactose, chitin and N-acetyl derivatives, with the exception of GalNAc. ACL-II is stable for up to 65 °C for 30 min, with a better stability at a pH range of 2 to 6. In contrast, ACL-I displays a strong mitogenic and cytotoxic effect.

摘要

从海洋海绵 Axinella corrugata（ACL-II）中纯化的凝集素 II 通过与兔血红细胞基质结合的亲和层析，然后在 Ultrogel AcA 44 柱上进行凝胶过滤进行纯化。通过 SDS-PAGE 和 FPLC 在 Superose 12 HR 柱上分别估计，纯化的 ACL-II 是一种 Mr 为 80 kDa 和 78 kDa 的凝集素。ACL-II 主要凝集天然兔红细胞，并且这种血凝活性不依赖于 Ca(2+)、Mg(2+)和 Mn(2+)，但受 d-半乳糖、几丁质和 N-乙酰衍生物的抑制，除 GalNAc 外。ACL-II 在高达 65°C 的温度下稳定 30 分钟，在 pH 值为 2 至 6 的范围内具有更好的稳定性。相比之下，ACL-I 表现出强烈的有丝分裂和细胞毒性作用。