大 VLDL 颗粒在代谢综合征中的优势，通过排阻液相色谱法检测到。
Predominance of large VLDL particles in metabolic syndrome, detected by size exclusion liquid chromatography.
机构信息
Laboratory of Lipids and Lipoproteins, Department of Clinical Biochemistry, Faculty of Pharmacy and Biochemistry, INFIBIOC, University of Buenos Aires, Argentina.
出版信息
Clin Biochem. 2012 Mar;45(4-5):293-7. doi: 10.1016/j.clinbiochem.2011.12.013. Epub 2012 Jan 3.
OBJECTIVE
To study size heterogeneity of triglyceride rich lipoproteins (TRL) in metabolic syndrome (MS).
DESIGN AND METHODS
Thirty MS patients and 14 healthy subjects were included. In fasting serum we measured: lipid profile, free fatty acids (FFA) and adiponectin; TRL were isolated (d<1.006 g/mL) and analysis by size exclusion HPLC followed by UV detection was performed; each subfraction was expressed as percentage of total TRL.
RESULTS
MS patients, even those with normal triglycerides, presented higher proportion of very large VLDL (90 nm diameter) and large VLDL (60 nm) and slightly lower of typical VLDL (37 nm) (p<0.04); increased FFA (p=0.04) and lower adiponectin (p=0.001). FFA correlated with large VLDL% (r=0.58; p=0.003), independently of insulin-resistance and waist. Furthermore, the lower the adiponectin, the greater the predominance of large VLDL (r=-0.40; p=0.04).
CONCLUSION
MS was associated with large VLDL, described as more atherogenic beyond triglyceride levels. Size exclusion HPLC would represent a useful tool for assessing subfractions' lipoprotein profile.
目的
研究代谢综合征（MS）患者中富含甘油三酯的脂蛋白（TRL）的大小异质性。
设计和方法
纳入 30 名 MS 患者和 14 名健康受试者。在空腹血清中，我们测量了：血脂谱、游离脂肪酸（FFA）和脂联素；分离 TRL（d<1.006 g/mL），并通过大小排阻 HPLC 进行分析，随后通过紫外检测进行分析；每个亚组分均表示为总 TRL 的百分比。
结果
即使甘油三酯正常，MS 患者也表现出更大比例的超大 VLDL（90nm 直径）和大 VLDL（60nm），而典型 VLDL（37nm）的比例略低（p<0.04）；FFA 升高（p=0.04），脂联素降低（p=0.001）。FFA 与大 VLDL%相关（r=0.58；p=0.003），与胰岛素抵抗和腰围无关。此外，脂联素越低，大 VLDL 的优势越大（r=-0.40；p=0.04）。
结论
MS 与大 VLDL 相关，除了甘油三酯水平外，其被描述为更具动脉粥样硬化性。大小排阻 HPLC 将成为评估脂蛋白谱亚组分的有用工具。
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