Börsch D, Westhoff P
Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, FRG.
FEBS Lett. 1990 Oct 29;273(1-2):111-5. doi: 10.1016/0014-5793(90)81063-t.
The primary structure of NADP-dependent malic enzyme (NADP-ME) of the dicotyledonous C4 plant Flaveria trinervia was determined from sequence analysis of a cDNA clone containing the complete coding region. Comparison of the mature F. trinervia NADP-ME with the maize enzyme reveals extensive sequence similarity. In contrast, no significant similarity can be detected between the putative transit peptides of the two enzymes. This suggests that the corresponding parts of the genes arose independently from each other during evolution of mono- and dicotyledonous C4 plants.
通过对一个包含完整编码区的cDNA克隆进行序列分析，确定了双子叶C4植物三脉叶黄菊（Flaveria trinervia）中依赖于NADP的苹果酸酶（NADP-ME）的一级结构。将成熟的三脉叶黄菊NADP-ME与玉米中的该酶进行比较，发现它们在序列上有广泛的相似性。相比之下，在这两种酶的假定转运肽之间未检测到显著的相似性。这表明在单子叶和双子叶C4植物的进化过程中，这两个基因的相应部分是彼此独立产生的。
