正常小鼠淋巴结中的淋巴祖细胞在体外和体内可分别分化为 NK 细胞和 T 细胞。

Lymphoid progenitors in normal mouse lymph nodes develop into NK cells and T cells in vitro and in vivo.

机构信息

Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.

出版信息

Exp Hematol. 2012 May;40(5):401-6. doi: 10.1016/j.exphem.2012.01.009. Epub 2012 Jan 21.

DOI:10.1016/j.exphem.2012.01.009

Abstract

We have identified a population of normal mouse LN cells, termed LN lymphoid progenitor (LNLP), resembling common lymphoid progenitor (CLP) in the bone marrow. LNLPs lack lineage markers and express CD127, low levels of CD117 (c-Kit), and Sca-1, but lack fms-related tyrosine kinase 3. They efficiently differentiate in vitro into natural killer (NK) cells and T cells, but not mature B cells. LNLPs injected into nonirradiated lymphopenic mice that have no LN develop into mostly splenic T cells with low numbers of NK cells and B cells. When injected into irradiated mice, they generate NK cells and T cells, but not B cells, in the LN. By contrast, bone marrow CLPs develop into mostly B cells with very small numbers of T and NK cells in recipients' spleen and LN. LNLPs have NK and T-cell potentials, but little B-cell potential, and they can develop into NK cells within the LN of normal mice, but their contribution to the T-cell lineage is unknown.

摘要

我们已经鉴定出一群正常的小鼠 LN 细胞，称为 LN 淋巴祖细胞（LNLP），类似于骨髓中的普通淋巴祖细胞（CLP）。LNLPs 缺乏谱系标志物，表达 CD127、低水平的 CD117（c-Kit）和 Sca-1，但缺乏 fms 相关酪氨酸激酶 3。它们在体外有效地分化为自然杀伤（NK）细胞和 T 细胞，但不能分化为成熟 B 细胞。将 LNLPs 注入没有 LN 的非辐射性淋巴缺失小鼠中，大多数会分化为具有少量 NK 细胞和 B 细胞的脾 T 细胞。当注入辐射小鼠时，它们会在 LN 中产生 NK 细胞和 T 细胞，但不会产生 B 细胞。相比之下，骨髓 CLPs 会在受者的脾和 LN 中分化为主要是 B 细胞，只有很少数量的 T 和 NK 细胞。LNLPs 具有 NK 和 T 细胞的潜力，但 B 细胞的潜力很小，它们可以在正常小鼠的 LN 中发育为 NK 细胞，但它们对 T 细胞谱系的贡献尚不清楚。