Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, Peoples' Republic of China.
Biol Trace Elem Res. 2012 Jun;147(1-3):142-8. doi: 10.1007/s12011-012-9329-5. Epub 2012 Jan 27.
An experiment was conducted to investigate the bioavailability of organic copper (Cu) proteinate relative to inorganic Cu sulfate for broiler chicks fed a conventional corn-soybean meal basal diet. A total of 320 day-old Arbor Acres commercial male chicks were assigned to one of five treatments in a completely randomized design involving a 2 × 2 factorial arrangement with two levels of added Cu (125 or 250 mg Cu/kg) and two Cu sources (Cu proteinate and Cu sulfate) plus a control with no added Cu for an experimental phase of 42 days. Plasma and liver tissue samples were collected at both 21 and 42 days of age, and bile samples were also obtained at 42 days of age for Cu analyses. The Cu concentrations in liver and bile increased linearly (P < 0.001) on both days 21 and 42 as dietary Cu levels increased. No significant (P > 0.17) linear regression relationships were observed between plasma Cu concentrations on days 21 and 42 or log10 liver Cu concentration on day 21 and daily analyzed Cu intake. Therefore, based on the slope ratios from multiple linear regressions of log10 liver and bile Cu concentrations with daily analyzed Cu intake on day 42, when Cu sulfate was set as 100%, the estimated relative bioavailability values of Cu proteinate were 78.8% for log10 liver Cu concentration and 79.3% for log10 bile Cu concentration, respectively. There was no significant (P > 0.08) difference in bioavailability between Cu proteinate and Cu sulfate for broilers chicks in this experiment.
一项实验旨在研究有机铜(Cu)蛋白盐相对于无机 Cu 硫酸盐对于以常规玉米-豆粕基础日粮喂养的肉鸡的生物利用率。总共 320 只 1 日龄 Arbor Acres 商业雄性小鸡被分配到 5 种处理中的一种,采用完全随机设计,涉及添加 Cu(125 或 250 mg/kg)和 2 种 Cu 源(Cu 蛋白盐和 Cu 硫酸盐)的 2×2 因子排列,外加一个不添加 Cu 的对照组,实验阶段为 42 天。在第 21 天和第 42 天收集血浆和肝脏组织样本,在第 42 天还收集胆汁样本进行 Cu 分析。在第 21 天和第 42 天,随着日粮 Cu 水平的增加,肝脏和胆汁中的 Cu 浓度呈线性增加(P < 0.001)。在第 21 天和第 42 天,血浆 Cu 浓度之间或第 21 天的 log10 肝脏 Cu 浓度与每日分析的 Cu 摄入量之间没有观察到显著的(P > 0.17)线性回归关系。因此,基于第 42 天每日分析的 Cu 摄入量与 log10 肝脏和胆汁 Cu 浓度的多元线性回归斜率比,当 Cu 硫酸盐设为 100%时,Cu 蛋白盐的估计相对生物利用率分别为 log10 肝脏 Cu 浓度的 78.8%和 log10 胆汁 Cu 浓度的 79.3%。在这项实验中,肉鸡对 Cu 蛋白盐和 Cu 硫酸盐的生物利用率没有显著差异(P > 0.08)。