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基于合成肽的环境敏感荧光传感器。

Environmentally sensitive fluorescent sensors based on synthetic peptides.

机构信息

Biosensor group, CNRS/Université de Strasbourg, IREBS, Parc d'Innovation, Boulevard Sébastien Brandt, BP 10413, 67412 Illkirch cedex, France.

出版信息

Sensors (Basel). 2010;10(4):3126-44. doi: 10.3390/s100403126. Epub 2010 Mar 31.

DOI:10.3390/s100403126
PMID:22319290
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3274215/
Abstract

Biosensors allow the direct detection of molecular analytes, by associating a biological receptor with a transducer able to convert the analyte-receptor recognition event into a measurable signal. We review recent work aimed at developing synthetic fluorescent molecular sensors for a variety of analytes, based on peptidic receptors labeled with environmentally sensitive fluorophores. Fluorescent indicators based on synthetic peptides are highly interesting alternatives to protein-based sensors, since they can be synthesized chemically, are stable, and can be easily modified in a site-specific manner for fluorophore coupling and for immobilization on solid supports.

摘要

生物传感器允许通过将生物受体与能够将分析物-受体识别事件转换为可测量信号的换能器相关联，从而直接检测分子分析物。我们综述了最近旨在开发基于用环境敏感荧光团标记的肽受体的各种分析物的合成荧光分子传感器的工作。基于合成肽的荧光指示剂是基于蛋白质的传感器的非常有吸引力的替代品，因为它们可以通过化学合成，稳定，并且可以通过荧光团偶联和固定在固体载体上的方式进行特异性修饰，从而可以轻松修饰。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/5baefb19537b/sensors-10-03126f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/f95d1329d50c/sensors-10-03126f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/f87e2e1a2c9f/sensors-10-03126f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/c7b7fb9de95d/sensors-10-03126f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/a77d3eb3417b/sensors-10-03126f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/1378451f46e3/sensors-10-03126f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/5baefb19537b/sensors-10-03126f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/f95d1329d50c/sensors-10-03126f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/f87e2e1a2c9f/sensors-10-03126f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/c7b7fb9de95d/sensors-10-03126f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/a77d3eb3417b/sensors-10-03126f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/1378451f46e3/sensors-10-03126f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc05/3274215/5baefb19537b/sensors-10-03126f6.jpg

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本文引用的文献

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一种具有笼型荧光性质的半胱氨酸反应性小分子光交联剂：荧光成像/串联质谱法测定组合筛选肽的结合位点。
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近年来，基于蛋白质的荧光生物传感器的构建策略取得了新进展。
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A general screening strategy for peptide-based fluorogenic ligands: probes for dynamic studies of PDZ domain-mediated interactions.基于肽的荧光配体的通用筛选策略：用于PDZ结构域介导相互作用动态研究的探针
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Inhibitory study of protein arginine methyltransferase 1 using a fluorescent approach.使用荧光方法对蛋白质精氨酸甲基转移酶1进行抑制研究。
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