内膜蛋白 YhiM 对大肠杆菌（E. coli）在酸性条件下的生存是必需的。
The inner membrane protein, YhiM, is necessary for Escherichia coli (E. coli) survival in acidic conditions.
机构信息
Department of Biology, Wabash College, 301 W. Wabash Avenue, Crawfordsville, IN 47933, USA.
出版信息
Arch Microbiol. 2012 Jul;194(7):637-41. doi: 10.1007/s00203-012-0798-x. Epub 2012 Feb 12.
Escherichia coli must be able to survive extreme acidic conditions. We were interested in determining the role of the inner membrane protein YhiM in survival in acidic conditions. Previous data demonstrated that the yhiM gene was upregulated in acidic conditions (Tucker et al. in J Bacteriol. 184:6551-6558, 2002). We therefore tested tn10 insertions into the yhiM gene for their ability to survive at low pH (pH 2.5). We show that YhiM was required for survival at pH 2.5. We also tested the YhiM dependence of the different acid resistance pathways. YhiM was required for the RpoS, glutamine and lysine-dependent acid resistance pathways. In contrast, YhiM was not required for the arginine-dependent acid resistance pathway.
大肠杆菌必须能够在极端酸性条件下生存。我们感兴趣的是确定内膜蛋白 YhiM 在酸性条件下生存中的作用。先前的数据表明，yhiM 基因在酸性条件下（Tucker 等人，在 J Bacteriol. 184:6551-6558, 2002）上调。因此，我们测试了 tn10 插入 yhiM 基因的能力，以观察它们在低 pH（pH 2.5）下的生存能力。我们表明 YhiM 是在 pH 2.5 下生存所必需的。我们还测试了不同的酸抗性途径对 YhiM 的依赖性。RpoS、谷氨酰胺和赖氨酸依赖的酸抗性途径都需要 YhiM。相比之下，YhiM 并不需要依赖精氨酸的酸抗性途径。
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