瘦素在结直肠癌组织中的细胞信号转导通路：初步结果
Cellular signal transduction pathways by leptin in colorectal cancer tissue: preliminary results.
作者信息
Nowakowska-Zajdel Ewa, Mazurek Urszula, Stachowicz Malgorzata, Niedworok Elzbieta, Fatyga Edyta, Muc-Wierzgoń Małgorzata
机构信息
Department of Internal Diseases, Medical University of Silesia, Żeromskiego 7 Street, 41-902 Bytom, Poland.
出版信息
ISRN Endocrinol. 2011;2011:575397. doi: 10.5402/2011/575397. Epub 2011 Mar 28.
The aim of the study was to analyse genes typing with the use of the oligonucleotide microarray technique (HG-U133A, Affymetrix) differentiating colorectal cancer tissues from tissues assessed histopathologically as healthy ones among a panel of 91 mRNA of genes encoding proteins involved in activation of cellular signal transduction pathways by leptin. Frozen tumor specimens from 11 colon cancer patients in various stages of clinical progression of the disease in an I-IV stage scale according to the TNM staging were used in molecular tests. Among the genes participating in the cascade of signal transfer in cell activated by leptin, the following ones: AKT1, STAT3, MCL1 were qualified as differentiating stage I and II and VEGFC, CCNDI the encoding genes respectively as differentiating III and IV stage neoplasm. It is necessary to extend studies of analysis of cellular signal transduction pathways by leptin in colorectal cancer initiation and transformation processes.
本研究的目的是利用寡核苷酸微阵列技术（HG-U133A，Affymetrix）对一组91种编码参与瘦素激活细胞信号转导途径的蛋白质的基因进行基因分型，以区分结直肠癌组织和经组织病理学评估为健康的组织。根据TNM分期，对11例处于疾病临床进展I-IV期不同阶段的结肠癌患者的冷冻肿瘤标本进行分子检测。在参与瘦素激活细胞信号转导级联反应的基因中，AKT1、STAT3、MCL1被鉴定为区分I期和II期的基因，而VEGFC、CCNDI的编码基因分别被鉴定为区分III期和IV期肿瘤的基因。有必要扩大对瘦素在结直肠癌发生和转化过程中细胞信号转导途径分析的研究。
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