Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, College of Forestry, Southwest Forestry University, Kunming, China.
PLoS One. 2012;7(2):e32291. doi: 10.1371/journal.pone.0032291. Epub 2012 Feb 23.
The pine shoot beetle Tomicus yunnanensis (Coleoptera: Scolytinae) is an economically important pest of Pinus yunnanensis in southwestern China. Developed resistance to insecticides due to chemical pesticides being used for a long time is a factor involved in its serious damage, which poses a challenge for management. In addition, highly efficient adaptation to divergent environmental ecologies results in this pest posing great potential threat to pine forests. However, the molecular mechanisms remain unknown as only limited nucleotide sequence data for this species is available.
METHODOLOGY/PRINCIPAL FINDINGS: In this study, we applied next generation sequencing (Illumina sequencing) to sequence the adult transcriptome of T. yunnanensis. A total of 51,822,230 reads were obtained. They were assembled into 140,702 scaffolds, and 60,031 unigenes. The unigenes were further functionally annotated with gene descriptions, Gene Ontology (GO), Clusters of Orthologous Groups (COG), and Kyoto Encyclopedia of Genes and Genome (KEGG). In total, 80,932 unigenes were classified into GO, 13,599 unigenes were assigned to COG, and 33,875 unigenes were found in KO categories. A biochemical pathway database containing 219 predicted pathways was also created based on the annotations. In depth analysis of the data revealed a large number of genes related to insecticides resistance and heat shock protein genes associated with environmental stress.
CONCLUSIONS/SIGNIFICANCE: The results facilitate the investigations of molecular resistance mechanisms to insecticides and environmental stress. This study lays the foundation for future functional genomics studies of important biological questions of this pest.
松梢小卷蛾（Tomicus yunnanensis）（鞘翅目：小蠹科）是中国西南地区云南松的一种重要经济害虫。由于长期使用化学农药，导致其对杀虫剂产生了抗性，这是其严重危害的一个因素，给防治工作带来了挑战。此外，该害虫高度适应不同的环境生态，对松林构成了巨大的潜在威胁。然而，由于该物种的核苷酸序列数据有限，其分子机制仍不清楚。
方法/主要发现：在本研究中，我们应用下一代测序（Illumina 测序）对松梢小卷蛾成虫转录组进行测序。共获得 51822230 条reads，组装成 140702 个 scaffolds 和 60031 个 unigenes。进一步对 unigenes 进行基因描述、基因本体论（GO）、直系同源簇（COG）和京都基因与基因组百科全书（KEGG）功能注释。共有 80932 个 unigenes被分类到 GO，13599 个 unigenes被分配到 COG，33875 个 unigenes在 KO 类别中。还根据注释创建了包含 219 个预测途径的生化途径数据库。对数据的深入分析揭示了大量与杀虫剂抗性和与环境应激相关的热休克蛋白基因相关的基因。
结论/意义：结果有助于研究昆虫抗药性的分子机制和环境应激相关的基因。本研究为该害虫重要生物学问题的功能基因组学研究奠定了基础。
