奥利司他对大鼠脑脂质过氧化、钠钾ATP酶、谷胱甘肽和血清素的影响。

Effect of orlistat on lipid peroxidation, Na+, K+ ATPase, glutathione and serotonin in rat brain.

作者信息

Calderón Guzmán D, Hernández García E, Juárez Jacobo A, Segura Abarca L, Barragán Mejía G, Rodríguez Pérez R, Juárez Olguín H

机构信息

Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), México City, Mexico.

出版信息

Proc West Pharmacol Soc. 2011;54:73-7.

PMID:22423586

Abstract

The aim of this study was to evaluate the effect of orlistat, a drug used in weight loss, on 5-HT and indicators of oxidative stress in rat brain. Orlistat, 12 mg/kg was administered to Wistar rats as single dose or successive doses on 3 consecutive days. Blood glucose and oxidative stress indicators were detected by measurement of lipid peroxidation, Na+, K+ ATPase, glutathione and serotonin levels using previous validated methods. The levels of glucose decreased in rats receiving successive doses. The activity of Na+, K+ ATPase and total ATPase was reduced in rats receiving successive doses, while the level of lipid peroxidation increased slightly in both groups. Glutathione underwent significant reduction in the successive doses group (p < 0.05). 5-HT increased significantly after single dose treatment (p < 0.05). Orlistat can induce pro-oxidant effects in the brain due to alteration of serotonergic metabolism and the reduction of glutathione.

摘要

本研究的目的是评估用于减肥的药物奥利司他对大鼠脑内5-羟色胺（5-HT）及氧化应激指标的影响。将12毫克/千克的奥利司他以单剂量或连续3天的连续剂量给予Wistar大鼠。使用先前验证的方法，通过测量脂质过氧化、Na+、K+三磷酸腺苷酶（ATPase）、谷胱甘肽和血清素水平来检测血糖和氧化应激指标。接受连续剂量的大鼠血糖水平下降。接受连续剂量的大鼠中Na+、K+ ATPase和总ATPase的活性降低，而两组中脂质过氧化水平均略有升高。连续剂量组中谷胱甘肽显著减少（p < 0.05）。单剂量治疗后5-HT显著增加（p < 0.05）。由于血清素能代谢的改变和谷胱甘肽的减少，奥利司他可在脑内诱导促氧化作用。