[通过酶联免疫吸附测定法检测母血和新生儿血清中针对Ia、Ib、II和III型B族链球菌的型特异性抗体]

[Type-specific antibody to type Ia, Ib, II and III group B Streptococcus in maternal and neonatal sera measured by ELISA].

作者信息

Sugiyama M, Kojima M, Hori Y, Simizu S, Sugiyama A, Ooe Y, Nishiyama Y

机构信息

Department of Obstetrics and Gynecology, Meijo Hospital, Nagoya.

出版信息

Kansenshogaku Zasshi. 1990 Sep;64(9):1232-8. doi: 10.11150/kansenshogakuzasshi1970.64.1232.

DOI:10.11150/kansenshogakuzasshi1970.64.1232

PMID:2243195

Abstract

In order to determine the type-specific antibody to group B streptococcus (GBA) type Ia, Ib, II and III, the ELISA system was established in Research laboratories, Toyo Jozo Co., Ltd. We assayed type-specific antibody by this ELISA system in both maternal and neonatal (or cord) sera. The cut off levels were determined by the antibody levels of maternal and neonatal sera of 26 infected cases and 90 GBS carriers, that type Ia, Ib, II were 0.20 and type III was 0.15. Prevalence of type-specific antibody levels were studied in 356 maternal sera (14 affected cases, 100 GBS carriers and 242 non carriers). Antibody levels were positive in 47.2% of maternal sera to type Ia, 34.0% to type Ib, 46.9% to type II and 45.5% to type III. Antibody levels to type Ia, Ib, II and III were positive, respectively, in 100%, 88.2%, 25.0% and 42.9% of the sera of carrier mothers whose infants were not affected. Antibody levels in 50 pair sera of maternal and cord blood were well correlated.

摘要

为了检测针对B族链球菌（GBA）Ia、Ib、II和III型的型特异性抗体，东洋酿造株式会社研究实验室建立了酶联免疫吸附测定（ELISA）系统。我们用该ELISA系统检测了母体血清和新生儿（或脐带）血清中的型特异性抗体。通过对26例感染病例和90例B族链球菌携带者的母体和新生儿血清抗体水平进行测定，确定Ia、Ib、II型的临界值为0.20，III型为0.15。研究了356份母体血清（14例感染病例、100例B族链球菌携带者和242例非携带者）中型特异性抗体水平的流行情况。Ia型母体血清抗体阳性率为47.2%，Ib型为34.0%，II型为46.9%，III型为45.5%。在婴儿未受感染的携带者母亲血清中，Ia、Ib、II和III型抗体水平分别为100%、88.2%、25.0%和42.9%呈阳性。50对母血和脐带血血清中的抗体水平具有良好的相关性。