豆浆：免疫印迹法中一种有效且廉价的封闭剂。
Soymilk: an effective and inexpensive blocking agent for immunoblotting.
机构信息
School of Biological Sciences, Illinois State University, Normal, IL 61790, USA.
出版信息
Anal Biochem. 2012 Jul 1;426(1):22-3. doi: 10.1016/j.ab.2012.03.028. Epub 2012 Apr 4.
Abstract
Blocking efficacy of whole soymilk, nonfat soymilk, SuperBlock, and nonfat milk was evaluated by performing standard protein immunoblotting procedures on both purified protein and crude nuclear extracts from HEK 293 cells. Nonfat soymilk was found to have superior blocking efficacy compared with other blocking agents in terms of high signal-to-noise ratio with the shortest blocking times. In addition, the presence of low concentrations of the detergent Tween 20 (0.05-0.1%, v/v) in the wash buffer as well as antibody incubations significantly lessened the background compared with including only the detergent during wash steps.
摘要
通过对 HEK 293 细胞的纯化蛋白和粗核提取物进行标准蛋白免疫印迹程序，评估了全豆乳、无脂豆乳、SuperBlock 和无脂牛奶的阻断效果。与其他阻断剂相比，无脂豆乳在具有最高信噪比的情况下，阻断时间最短，阻断效果更好。此外，在洗涤缓冲液和抗体孵育中加入低浓度的去污剂吐温 20（0.05-0.1%，v/v），与仅在洗涤步骤中加入去污剂相比，背景显著降低。
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