前催化状态下的 II 型内含子的晶体结构。
Crystal structure of a group II intron in the pre-catalytic state.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, San Diego, California, USA.
出版信息
Nat Struct Mol Biol. 2012 Apr 8;19(5):555-7. doi: 10.1038/nsmb.2270.
Abstract
Group II introns are self-splicing catalytic RNAs that are thought to be ancestral to the spliceosome. Here we report the 3.65-Å crystal structure of the group II intron from Oceanobacillus iheyensis in the pre-catalytic state. The structure reveals the conformation of the 5' splice site in the catalytic core and represents the first structure of an intron prior to the first step of splicing.
摘要
内含子 II 是自我剪接的催化 RNA，被认为是剪接体的前身。在这里，我们报告了来自海洋芽孢杆菌的内含子 II 在预催化状态下的 3.65Å 晶体结构。该结构揭示了催化核心中 5'剪接位点的构象，代表了剪接第一步之前的第一个内含子结构。
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