白细胞介素 17A 和白细胞介素 17F 基因多态性与中国人群变应性鼻炎及其合并哮喘发病的相关性研究。
Association between polymorphisms in cytokine genes IL-17A and IL-17F and development of allergic rhinitis and comorbid asthma in Chinese subjects.
机构信息
Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, PR China.
出版信息
Hum Immunol. 2012 Jun;73(6):647-53. doi: 10.1016/j.humimm.2012.03.010. Epub 2012 Apr 13.
BACKGROUND
Th17 cell lineage, a distinct pro-inflammatory lineage characterized by preferential synthesis of cytokines IL-17A and IL-17F, is thought to play an important role in the pathogenesis of allergic rhinitis (AR).
OBJECTIVES
Our aim was to investigate whether polymorphisms in and around IL-17A and IL-17F genes are associated with AR and comorbid asthma.
METHODS
A case-control comparison was performed in a cohort of 279 AR patients, 197 allergic rhinitis with asthma (AR-A) patients and 281 control Chinese subjects, to investigate associations between 19 tagging single-nucleotide polymorphisms (SNPs) in IL-17A and IL-17F gene regions and manifestation of AR or AR-A. Genotyping was performed using the Sequenom MassARRAY platform.
RESULTS
SNP rs3819024 in IL-17A gene, intergenic SNPs rs1892280 and rs10807439 were specifically associated with AR protective or risk effects, while rs3819024 in IL-17A gene, intergenic SNP rs13192563 in IL-17F gene were associated with AR-A protective or risk effects. Haplotype analysis showed significant AR risk in haplotype AA (rs1892280G-rs13192563A) and AR protective effect in haplotype GT (rs7758579A-rs11966760T); the haplotype AT(rs7758579-rs11966760) were considered AR-A risk.
CONCLUSIONS
Our findings preliminarily indicate IL17A and IL17F SNPs, and some intergenic variants have the potential association with AR and comorbid asthma in Chinese population.
背景
Th17 细胞谱系是一种独特的促炎谱系，其特征是优先合成细胞因子 IL-17A 和 IL-17F，被认为在过敏性鼻炎（AR）的发病机制中发挥重要作用。
目的
我们旨在研究 IL-17A 和 IL-17F 基因内和周围的多态性是否与 AR 和伴发哮喘有关。
方法
在 279 例 AR 患者、197 例过敏性鼻炎伴哮喘（AR-A）患者和 281 例对照中国受试者的队列中进行病例对照比较，以研究 IL-17A 和 IL-17F 基因区域的 19 个标记单核苷酸多态性（SNP）与 AR 或 AR-A 表现之间的关联。采用Sequenom MassARRAY 平台进行基因分型。
结果
IL-17A 基因中的 SNP rs3819024、基因间 SNP rs1892280 和 rs10807439 与 AR 保护或风险效应特异性相关，而 IL-17A 基因中的 SNP rs3819024、IL-17F 基因中的基因间 SNP rs13192563 与 AR-A 保护或风险效应相关。单体型分析显示，单体型 AA（rs1892280G-rs13192563A）具有显著的 AR 风险，单体型 GT（rs7758579A-rs11966760T）具有 AR 保护作用；单体型 AT（rs7758579-rs11966760）被认为是 AR-A 的风险。
结论
我们的研究结果初步表明，IL17A 和 IL17F SNP 以及一些基因间变异可能与中国人群的 AR 和伴发哮喘有关。
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