筛查中国雷州半岛栖息的滨蟹的毒性和麻痹性贝类毒素抗性。

Screening for toxicity and resistance to paralytic shellfish toxin of shore crabs inhabiting at Leizhou peninsula, China.

机构信息

College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.

出版信息

Mar Environ Res. 2012 Jul;78:48-52. doi: 10.1016/j.marenvres.2012.04.002. Epub 2012 Apr 14.

DOI:10.1016/j.marenvres.2012.04.002

Abstract

The situation of the environment contaminated by paralytic shellfish toxin (PST) in Leizhou peninsula, China, has attracted more attention since seafood poisoning occurred occasionally. In this study, we examined the toxicities of shore crab Leptodius exaratus, Thalamita crenata and Metopograpsus latifrons by mouse assay, resistance to PST by lethal test injection with PST, and discussed the toxicity neutralization of their hemolymph. The results showed 12% of shore crabs possessed toxicity of 4.3-4.4 MU/g. The 100% lethal dose of PST for M. latifrons was about 2 times of those for the other two crab species. The hemolymphs of the crabs were all able to neutralize PST and tetrodotoxin (TTX) toxicity in different extent. The above results indicate shore crabs at this area are exposed to an environment potentially contaminated with PST and/or TTX, and the toxicity neutralizing efficacy of their hemolymph directly affects their resistance to the toxins.

摘要

中国雷州半岛的麻痹性贝类毒素（PST）污染情况引起了越来越多的关注，因为这里偶尔会发生海鲜中毒事件。在这项研究中，我们通过小鼠检测法检测了滨蟹（Leptodius exaratus）、波纹巴非蛤（Thalamita crenata）和锈斑蟳（Metopograpsus latifrons）的毒性，以及它们对 PST 的抗毒性（通过 PST 致死性注射测试），并讨论了它们血淋巴的毒性中和作用。结果表明，12%的滨蟹具有 4.3-4.4 MU/g 的毒性。PST 对锈斑蟳的 100%致死剂量约为另外两种蟹类的两倍。这些螃蟹的血淋巴均能在不同程度上中和 PST 和河豚毒素（TTX）的毒性。上述结果表明，该地区的滨蟹暴露在可能受到 PST 和/或 TTX 污染的环境中，其血淋巴的毒性中和效果直接影响它们对毒素的抵抗力。