人骨髓间充质干细胞与视网膜色素上皮细胞共培养诱导分化为神经样细胞
Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells.
作者信息
Yang Ling-Ling, Zhou Qing-Jun, Wang Yao, Wang Yi-Qiang
机构信息
State Key Laboratory Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao 266071, Shandong Province, China.
出版信息
Int J Ophthalmol. 2010;3(1):23-7. doi: 10.3980/j.issn.2222-3959.2010.01.06. Epub 2010 Mar 18.
AIM
To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC).
METHODS
Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC.
RESULTS
The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE.
CONCLUSION
Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
目的
检测视网膜细胞或提取物对骨髓间充质干细胞（BMSC）的分化作用。
方法
人胎儿BMSC预先用羧基荧光素琥珀酰亚胺酯（CFSE）标记，然后与经紫外线照射预处理的视网膜色素上皮（RPE）细胞按1:1比例共培养，诱导BMSC分化长达14天。在一些实验中，添加牛视网膜提取物（BRE）以检测视网膜成分对BMSC分化的潜在影响。此外，进行神经元特异性烯醇化酶（NSE）、巢蛋白和胶质纤维酸性蛋白（GFAP）免疫染色以确定BMSC的特性。
结果
结果表明，通过与RPE细胞共培养，胎儿BMSC分化为表达特殊神经元标志物巢蛋白、GFAP和NSE的神经样细胞。BRE可明显增加这些标志物的表达。
结论
视网膜来源的细胞和提取物可诱导BMSC分化为神经样细胞。
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