野菊变种白花蒿中的 6-甲氧基木犀草素通过下调 FcεRIα链表达抑制组胺释放和钙离子内流。

6-Methoxyluteolin from Chrysanthemum zawadskii var. latilobum suppresses histamine release and calcium influx via down-regulation of FcεRI α chain expression.

机构信息

Institute of Marine Life Science, Pukyong National University, Busan, 608-737, Korea.

出版信息

J Microbiol Biotechnol. 2012 May;22(5):622-7. doi: 10.4014/jmb.1111.11060.

DOI:10.4014/jmb.1111.11060

Abstract

Mast cells and basophils are important effector cells in immunoglobulin-E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed the inhibitory effects of 6-methoxyluteolin, isolated from Chrysanthemum zawadskii, in the FcεRI-mediated allergic reaction. We determined that 6-methoxyluteolin inhibited anti-FcεRI α chain antibody (CRA-1)-induced histamine release, as well as elevation of intracellular calcium concentration [Ca2+]i in a dose-dependent manner. Moreover, the inhibitory effects of 6-methoxyluteolin on the cell surface expression and the mRNA level of the FcεRI α chain were determined by flow cytometric analysis and reverse transcription-polymerase chain reaction (RTPCR), respectively. Therefore, these results show that 6- methoxyluteolin is a potent inhibitor of histamine release and calcium influx via down-regulation of the FcεRI α chain.

摘要

肥大细胞和嗜碱性粒细胞是免疫球蛋白-E（IgE）介导的过敏反应中的重要效应细胞。我们使用人嗜碱性粒细胞 KU812F 细胞，评估了从菊苣中分离得到的 6-甲氧基木犀草素在 FcεRI 介导的过敏反应中的抑制作用。我们发现 6-甲氧基木犀草素能够抑制抗 FcεRIα链抗体（CRA-1）诱导的组胺释放，以及细胞内钙离子浓度 [Ca2+]i 的升高，且呈剂量依赖性。此外，我们通过流式细胞术分析和逆转录-聚合酶链反应（RT-PCR）分别确定了 6-甲氧基木犀草素对 FcεRIα链的细胞表面表达和 mRNA 水平的抑制作用。因此，这些结果表明 6-甲氧基木犀草素是一种通过下调 FcεRIα链来抑制组胺释放和钙内流的有效抑制剂。