Department of Orthopaedic Surgery, Rouen Teaching Hospital Center, 1, rue de Germont, 76031 Rouen cedex, France.
Orthop Traumatol Surg Res. 2012 Jun;98(4):432-40. doi: 10.1016/j.otsr.2011.10.015. Epub 2012 May 10.
Double gloving is recommended in orthopedic surgery, notably in total hip arthroplasties (THA) to prevent contamination of the surgical site.
Systematic glove changes during the key phases of hip prosthesis implantation reduce the frequency of occult perforations and bacterial loading of glove surfaces.
During 29 THA implantation procedures, we evaluated the bacterial contamination of the outer glove surface and its perforation rate. Contaminations were sought by placing the gloved fingertips on blood geloses (incubation, 48 h at 37°C), and perforations were sought using a water test (NF EN 455-1).
One intervention was excluded from the study because an initial contamination was detected, leaving 28 cases analyzed. Fifteen interventions (53.6%) presented contaminated geloses (26 contaminated glove changes for 3.38% of the gloves used). These contaminations were found on the gloves of all of the gloved personnel, with no distinction as to the right or left side. Thirty-eight percent of the contaminations occurred during joint reduction, whereas the other surgical stages grouped 15-26% of the contaminations (P<0.05). Twenty-nine bacteria were identified: 62% coagulase-negative staphylococci (16% of which were methicillin-resistant). Twenty-eight perforations were identified (3.5% of the gloves used), 67.8% of which were located on the operator and 64.3% on the dominant side. Eighty percent of the perforations occurred during the "surgical incision" and the "cup and stem implantation" stages (respectively, 5.0% and 5.5% of the gloves used during the surgical time) (P<0.05), without being associated with an increased risk of bacterial contamination. At the 12-month clinical follow-up, no infectious complications were found. On the gloves worn by the 20 surgical team members contaminated during these 28 surgical procedures, replacing contaminated gloves with new sterile gloves rendered all the bacteriological samples of the subsequent surgical stages negative in 16 cases (80%).
Increasing the number of outer glove renewals, notably during certain surgical stages at risk for contamination (prosthesis reduction) or perforation (surgical incision/femoral cementing) can reduce the risk of contamination and perforation. The bacteria isolated suggest a cutaneous origin. Regularly changing gloves has resulted in a sterile state in 80% of cases. LEVEL OF EVIDENCE AND TYPE OF STUDY: Level III prospective diagnostic study.
在矫形外科手术中,尤其是在全髋关节置换术(THA)中,建议使用双层手套,以防止手术部位的污染。
在髋关节假体植入的关键阶段系统地更换手套可以降低手套表面隐性穿孔和细菌负荷的频率。
在 29 例 THA 植入手术中,我们评估了外层手套表面的细菌污染及其穿孔率。通过将戴手套的指尖放在血凝胶上(孵育,37°C 下 48 小时)来寻找污染,并用水测试(NF EN 455-1)来寻找穿孔。
由于最初检测到污染,排除了一项干预措施,留下了 28 例分析。15 例干预措施(53.6%)的凝胶呈污染状态(3.38%使用的手套中有 26 个污染的手套更换)。这些污染发生在所有戴手套的人员的手套上,没有左右侧的区别。38%的污染发生在关节复位过程中,而其他手术阶段则有 15-26%的污染(P<0.05)。鉴定出 29 种细菌:62%凝固酶阴性葡萄球菌(其中 16%为耐甲氧西林)。鉴定出 28 个穿孔(使用的手套的 3.5%),67.8%位于操作员侧,64.3%位于优势侧。80%的穿孔发生在“手术切口”和“杯和柄植入”阶段(分别占手术时间使用的手套的 5.0%和 5.5%)(P<0.05),但与细菌污染的风险增加无关。在 12 个月的临床随访中,未发现感染性并发症。在这 28 例手术中被污染的 20 名手术团队成员所戴的手套上,更换新的无菌手套后,在 16 例(80%)中使随后的手术阶段的所有细菌样本均为阴性。
增加外层手套的更换次数,特别是在某些有污染风险的手术阶段(假体复位)或穿孔(手术切口/股骨骨水泥)时,可以降低污染和穿孔的风险。分离出的细菌提示来自皮肤。定期更换手套可使 80%的情况下达到无菌状态。
三级前瞻性诊断研究。