从聚丙烯酰胺凝胶中分离蛋白质。

Isolation of proteins from polyacrylamide gels.

作者信息

Michalk Irene, Koristka Stefanie, Arndt Claudia, Bachmann Michael

机构信息

Carl Gustav Carus University TU Dresden, Dresden, Germany.

出版信息

Methods Mol Biol. 2012;869:427-31. doi: 10.1007/978-1-61779-821-4_35.

DOI:10.1007/978-1-61779-821-4_35

PMID:22585506

Abstract

Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.

摘要

为了产生包括单克隆抗体在内的抗体，给小鼠免疫需要微量蛋白质。在此，我们描述了一种在十二烷基硫酸钠聚丙烯酰胺凝胶电泳后从聚丙烯酰胺凝胶中快速分离蛋白质的方法，所获蛋白量足以用于动物免疫。