Ness SallyAnne L, Peters-Kennedy Jeanine, Schares Gereon, Dubey Jitender P, Mittel Linda D, Mohammed Hussni O, Bowman Dwight D, Felippe M Julia B, Wade Susan E, Shultz Nicole, Divers Thomas J
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
J Am Vet Med Assoc. 2012 Jun 1;240(11):1329-37. doi: 10.2460/javma.240.11.1329.
To describe the clinical, endoscopic, and serologic features of an outbreak of besnoitiosis in 2 donkey operations in northeastern Pennsylvania and to report the outcome of attempted treatment of 1 naturally infected individual.
Observational study.
29 donkeys (Equus asinus) in northeastern Pennsylvania.
Donkeys were examined for lesions suggestive of besnoitiosis in an outbreak investigation. Information was collected regarding the history and signalment of animals on each premises. Rhinolaryngoscopy was performed to identify nasopharyngeal and laryngeal lesions. Serum samples were collected for immunofluorescent antibody testing and immunoblotting for Besnoitia spp. Skin biopsy samples were obtained from 8 animals with lesions suggestive of besnoitiosis for histologic examination. Quantitative real-time PCR assay for Besnoitia spp was performed on tissue samples from 5 animals.
Besnoitiosis was confirmed in 6 of the 8 suspected cases. The most common lesion site was the nares, followed by the skin and sclera. Donkeys with clinical signs of disease had higher serum antibody titers and tested positive for a greater number of immunoblot bands than did donkeys without clinical signs of disease. All animals evaluated by PCR assay tested positive. Putative risk factors for disease included age and sex. Ponazuril was not effective at treating besnoitiosis in a naturally infected donkey.
Knowledge of clinical and serologic features of besnoitiosis in donkeys will assist clinicians in the diagnosis and prevention of this disease in donkey populations. Besnoitiosis may be an emerging disease of donkeys in the United States.
描述宾夕法尼亚州东北部两家养驴场发生的贝诺孢子虫病暴发的临床、内镜和血清学特征，并报告对1例自然感染个体的治疗结果。
观察性研究。
宾夕法尼亚州东北部的29头驴（ Equus asinus）。
在疫情调查中，对驴进行检查，以寻找提示贝诺孢子虫病的病变。收集每家养殖场动物的病史和特征信息。进行鼻咽喉镜检查以识别鼻咽部和喉部病变。采集血清样本进行免疫荧光抗体检测和针对贝诺孢子虫属的免疫印迹分析。从8头有提示贝诺孢子虫病病变的动物身上获取皮肤活检样本进行组织学检查。对5头动物的组织样本进行贝诺孢子虫属的定量实时PCR检测。
8例疑似病例中有6例确诊为贝诺孢子虫病。最常见的病变部位是鼻孔，其次是皮肤和巩膜。有临床疾病体征的驴血清抗体滴度更高，免疫印迹条带检测呈阳性的数量比无临床疾病体征的驴更多。所有接受PCR检测的动物均呈阳性。疾病的推定风险因素包括年龄和性别。泊那唑对自然感染的驴治疗贝诺孢子虫病无效。
了解驴贝诺孢子虫病的临床和血清学特征将有助于临床医生在驴群中诊断和预防该病。贝诺孢子虫病可能是美国驴的一种新出现的疾病。
