Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a La Victoria Km 0.6 C.P. 83304, Hermosillo, Sonora, Mexico.
Virology. 2012 Aug 15;430(1):73-80. doi: 10.1016/j.virol.2012.04.009. Epub 2012 May 18.
The aim of this study was to analyze the regulatory T cells (Tregs) induced by the porcine reproductive and respiratory syndrome virus (PRRSV) in pigs. Serum, blood, tonsil, and mediastinal lymph nodes' samples were obtained at different time post-infection (dpi). The frequencies of CD4(+)CD8(-)CD25(+)Foxp3(+), CD4(+)CD8(+)CD25(+)Foxp3(+), or CD4(-)CD8(+)CD25(+)Foxp3(+) phenotypes were determined in PBMC and lymph node cells, and cells producing IL-10 or TGF-β were analyzed. PRRSV increased the number of CD4(+)CD8(+)CD25(+)Foxp3(+) cells at 14 dpi, whereas CD4(+)CD8(-)CD25(+)Foxp3(+) remained constant until 28 dpi. Positive correlation exists between viremia and induced regulatory cells. CD4(+)CD8(+)CD25(+)Foxp3(+)-induced Treg cells were consistently observed in lymphoid tissues. Analysis of IL-10- and TGF-β-producing cell demonstrated that in response to PRRSV, CD4(+)CD8(-)Foxp3(low) and CD4(+)CD8(+)Foxp3(high) cells increase moderately the proportion of IL-10(+) cells. TGF-β was only observed in the CD4(+)CD8(+)Foxp3(high) population after PRRSV stimulation. In conclusion, PRRSV infection increases the frequency of Tregs with the phenotype CD4(+)CD8(+)CD25(+)Foxp3(high) and produces TGF-β.
本研究旨在分析猪繁殖与呼吸综合征病毒（PRRSV）诱导的调节性 T 细胞（Treg）。在感染后不同时间（dpi）采集血清、血液、扁桃体和纵隔淋巴结样本。在 PBMC 和淋巴结细胞中测定 CD4(+)CD8(-)CD25(+)Foxp3(+)、CD4(+)CD8(+)CD25(+)Foxp3(+)或 CD4(-)CD8(+)CD25(+)Foxp3(+)表型的频率，并分析产生 IL-10 或 TGF-β的细胞。PRRSV 在 14dpi 时增加 CD4(+)CD8(+)CD25(+)Foxp3(+)细胞数量，而 CD4(+)CD8(-)CD25(+)Foxp3(+)在 28dpi 时保持不变。病毒血症与诱导的调节性细胞之间存在正相关。在淋巴组织中始终观察到 CD4(+)CD8(+)CD25(+)Foxp3(+)诱导的 Treg 细胞。分析产生 IL-10 和 TGF-β的细胞表明，PRRSV 刺激后，CD4(+)CD8(-)Foxp3(low)和 CD4(+)CD8(+)Foxp3(high)细胞适度增加 IL-10(+)细胞的比例。TGF-β仅在 PRRSV 刺激后 CD4(+)CD8(+)Foxp3(high)群体中观察到。总之，PRRSV 感染增加了具有 CD4(+)CD8(+)CD25(+)Foxp3(high)表型的 Treg 频率，并产生 TGF-β。
