Kamitakahara Masanobu, Kimura Kenshiro, Ioku Koji
Graduate School of Environmental Studies, Tohoku University, Aoba-ku, Sendai, Japan.
Colloids Surf B Biointerfaces. 2012 Sep 1;97:236-9. doi: 10.1016/j.colsurfb.2012.03.033. Epub 2012 Apr 28.
Nanosized porous hydroxyapatite (HA) granules are expected to be useful as DNA and drug carriers for cells. We attempted to synthesize nanosized HA granules in an agarose gel by electrophoresis of calcium and phosphate ions. Wells were prepared on an agarose gel, and a CaCl(2) solution was placed in the wells on the positive side and a Na(2)HPO(4) solution was placed in the wells on the negative side. After a potential was applied, white precipitates appeared in the gel. The precipitates were calcium-deficient HA granules of approximately 300-600 nm in diameter. These granules were composed of fiber-like particles and had a porous structure. The diameters of the granules were decreased by an increase in the gel concentration. Hence, we successfully synthesized nanosized porous HA granules to be used as DNA and drug carriers.
纳米级多孔羟基磷灰石（HA）颗粒有望用作细胞的DNA和药物载体。我们试图通过钙和磷酸根离子的电泳在琼脂糖凝胶中合成纳米级HA颗粒。在琼脂糖凝胶上制备孔，将CaCl₂溶液置于正极一侧的孔中，将Na₂HPO₄溶液置于负极一侧的孔中。施加电势后，凝胶中出现白色沉淀。沉淀是直径约300 - 600 nm的缺钙HA颗粒。这些颗粒由纤维状颗粒组成，具有多孔结构。颗粒直径随凝胶浓度的增加而减小。因此，我们成功合成了用作DNA和药物载体的纳米级多孔HA颗粒。
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