[实验性牙合紊乱对大鼠髁突软骨中白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α表达的影响]
[Effect of experimentally created disordered occlusion on the expression of IL-1β, IL-6 and TNF-α in condylar cartilage of rats].
作者信息
Dai Kang, Jiao Kai, Wang Dong-sheng, Yu Shi-bin, Liu Xiao-dong, Wang Mei-qing
机构信息
Department of Oral Anatomy and Physiology, Fourth Military Medical University,Shanxi Province, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2012 Apr;21(2):121-6.
PURPOSE
To investigate the effect of the experimentally created malocclusion (ECDO) on the expression of IL-1β, IL-6 and TNF-α in mandibular condylar cartilage of Sprague Dawley (SD) rats.
METHODS
In the experimental group, the malocclusion was created by moving the first and third molars 0.8 mm away. HE and immunohistochemical staining were performed on the mandibular condyle at the end of the 8th or 12th week. Gene expression was analyzed by real-time PCR. The data was processed using SPSS12.0 software package.
RESULTS
Degenerative changes were found in the experimental group, the level of IL-1β was not significantly different among the groups (P>0.05), while the expression of IL-6 in 8-week ECDO subgroup was significantly higher than that in 8-week control subgroup (P=0.009), and the expression of TNF-α in 8-week ECDO subgroup was significantly higher than that in 8-week control subgroup (P=0.001) and 12-week ECDO subgroup (P=0.004).
CONCLUSIONS
At the early period, IL-6 and TNF-α, but not IL-1β, play an important role in the degenerative remodeling procedure in rat condylar cartilage induced by ECDO.
目的
探讨实验性错牙合畸形（ECDO）对Sprague Dawley（SD）大鼠下颌髁突软骨中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达的影响。
方法
在实验组，通过将第一和第三磨牙向远中移动0.8 mm制造错牙合畸形。在第8周或第12周结束时，对下颌髁突进行苏木精-伊红（HE）染色和免疫组织化学染色。采用实时荧光定量聚合酶链反应（PCR）分析基因表达。数据用SPSS12.0软件包处理。
结果
实验组出现退行性改变，各组间IL-1β水平差异无统计学意义（P>0.05），而8周ECDO亚组IL-6表达明显高于8周对照组亚组（P=0.009），8周ECDO亚组TNF-α表达明显高于8周对照组亚组（P=0.001）和12周ECDO亚组（P=0.004）。
结论
在早期，IL-6和TNF-α而非IL-1β在ECDO诱导的大鼠髁突软骨退行性重塑过程中起重要作用。
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