大瓣芹素 A-J，来自大瓣芹的生物碱。

Daphmacromines A-J, alkaloids from Daphniphyllum macropodum.

机构信息

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China.

出版信息

J Nat Prod. 2012 Jun 22;75(6):1076-82. doi: 10.1021/np200960z. Epub 2012 May 29.

DOI:10.1021/np200960z

PMID:22642560

Abstract

Ten new yuzurine-type Daphniphyllum alkaloids, daphmacromines A-J (1-10), along with seven known alkaloids were isolated from the leaves and stems of Daphniphyllum macropodum. Their structures were elucidated by extensive spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry, and the structure of 1 was confirmed by single-crystal X-ray diffraction. The pesticidal and cytotoxic activities of the isolated alkaloids were evaluated in vitro against brine shrimp (Artemia salina) and five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, and SW480), respectively. This study also suggested structural revisions of oxodaphnigracine, oxodaphnigraciline, and epioxodaphnigraciline.

摘要

从大瓣瑞香的叶和茎中分离得到了 10 种新的乌苏里瑞香型二氢菲里定生物碱 daphmacromines A-J（1-10）以及 7 种已知生物碱。通过二维 NMR 光谱和质谱等广泛的光谱技术以及单晶 X 射线衍射确定了它们的结构。对分离得到的生物碱进行了体外杀虫和细胞毒性活性评估，分别针对卤虫（Artemia salina）和 5 个人类癌细胞系（HL-60、SMMC-7721、A-549、MCF-7 和 SW480）。本研究还对 oxodaphnigracine、oxodaphnigraciline 和 epioxodaphnigraciline 的结构进行了修订。