鸡视锥视觉色素之一的cDNA克隆及氨基酸序列分析

Cloning of cDNA and amino acid sequence of one of chicken cone visual pigments.

作者信息

Tokunaga F, Iwasa T, Miyagishi M, Kayada S

机构信息

Department of Physics, Faculty of Science, Tohoku University, Miyagi, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):1212-7. doi: 10.1016/s0006-291x(05)80915-5.

DOI:10.1016/s0006-291x(05)80915-5

PMID:2268324

Abstract

The chicken has four kinds of color visual pigments, in addition to rhodopsin. A chicken genomic DNA library was screened with cDNA of human red-sensitive pigment and a chicken genomic DNA fragment including rhodopsin exons 2, 3 and 4, and then a genomic DNA fragment encoding a visual pigment, possibly an iodopsin, was cloned. A cDNA library, constructed from chicken retina mRNA, was screened with the genomic DNA fragment and the cDNA of human red-sensitive pigment, and the cDNA encoding the pigment was cloned. The nucleotide sequence of this cDNA was similar to that of the human red-sensitive pigment, with identities of 78% for the nucleotide sequence and 84% for the amino acid sequence with human red-sensitive pigment.

摘要

除视紫红质外，鸡有四种颜色视觉色素。用人红色敏感色素的cDNA筛选鸡基因组DNA文库，得到一个包含视紫红质外显子2、3和4的鸡基因组DNA片段，随后克隆了一个编码视觉色素（可能是视锥视蛋白）的基因组DNA片段。用该基因组DNA片段和人红色敏感色素的cDNA筛选由鸡视网膜mRNA构建的cDNA文库，克隆了编码该色素的cDNA。该cDNA的核苷酸序列与人红色敏感色素的相似，核苷酸序列的同源性为78%，氨基酸序列与人类红色敏感色素的同源性为84%。