Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
Biochem Genet. 2012 Oct;50(9-10):770-83. doi: 10.1007/s10528-012-9519-z. Epub 2012 Jun 12.
We report here on the phylogenetic analysis, population substructure, and identification of molecular tags of 25 popular rice varieties and four landraces from different ecological belts of India employing a set of 52 simple sequence repeat (SSR) markers. Genetic analysis using the SSR markers categorized the genotypes into two major clusters, distributed according to their pedigree. Population structure analysis suggested that the optimum number of subpopulations was three (K = 3) in the popular varieties and landraces. At K = 5 the allelic distribution was much more similar to the phylogenetic dendrogram. The molecular diversity and population structure analysis indicated that there is not much variation among the popular rice cultivars of India. The study has identified SSR markers producing unique alleles, which should aid in the precise identification, maintenance, and genetic purity analysis of rice varieties.
我们在此报告了对 25 个流行的水稻品种和 4 个来自印度不同生态带的地方品种的系统发育分析、群体结构和分子标记的鉴定,使用了一套 52 个简单序列重复 (SSR) 标记。SSR 标记的遗传分析将基因型分为两个主要聚类,根据其系谱分布。种群结构分析表明,在流行品种和地方品种中,最佳亚群数为 3 个(K=3)。在 K=5 时,等位基因分布与系统发育树更相似。分子多样性和种群结构分析表明,印度流行的水稻品种之间没有太多的变异。这项研究已经确定了产生独特等位基因的 SSR 标记,这应该有助于水稻品种的精确鉴定、维护和遗传纯度分析。