[多形当归的高效液相色谱指纹图谱]
[Fingerprint of Angelica polymorpha by HPLC].
作者信息
Lu Jinqing, Wang Qin, Xu Jiaqi, He Dongli, Ruan Xiaoyun, Hu Xiaoyan
机构信息
Hubei University of Traditional Chinese Medicine, Wuhan 430065, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2012 Mar;37(6):832-5.
OBJECTIVE
To establish the HPLC fingerprint of Angelica polymorpha.
METHOD
The 10 batches of A. polymorpha were measured by HPLC with isoimperatorin as a reference substance and the chromatographic experients were performed on Kromasil 100A C18 column (4.6 mm x 250 mm, 5 microm), eluted with acetonitrile and water as mobile phase in gradient mode. The flow rate was 1.0 m x min(-1) and the detection wavelength was 254 nm.
RESULT
The common mode of the HPLC fingerprints were set up. There were 8 common peaks in the fingerprint of 10 samples, and the similarity of the 10 samples was more than 0.9.
CONCLUSION
The method is simple, accurate and have a good reaptability. The quality of A. polymorpha can be controlled effectively by the HPLC fingerprint.
目的
建立紫花前胡的高效液相色谱指纹图谱。
方法
以异欧前胡素为对照品，采用高效液相色谱法测定10批紫花前胡，色谱实验在Kromasil 100A C18柱（4.6 mm×250 mm，5μm）上进行，以乙腈和水为流动相进行梯度洗脱。流速为1.0 ml·min⁻¹，检测波长为254 nm。
结果
建立了高效液相色谱指纹图谱的共有模式。10个样品的指纹图谱中有8个共有峰，10个样品的相似度均大于0.9。
结论
该方法简便、准确、重复性好。高效液相色谱指纹图谱可有效控制紫花前胡的质量。
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