基于氟化物检测的γ-丁酰甜菜碱羟化酶荧光检测法的开发与应用。
Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.
机构信息
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
出版信息
Chembiochem. 2012 Jul 23;13(11):1559-63. doi: 10.1002/cbic.201200256. Epub 2012 Jun 22.
PMID:22730246
Abstract
Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.
摘要
用于加氧酶的氟化物检测
2-氧代戊二酸依赖性加氧酶 BBOX 催化肉碱生物合成的最后一步，是药物化学的靶标。我们报告称，BBBOX 可以羟基化氟代底物类似物，随后释放出氟离子，从而实现基于荧光的高效检测。
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