Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Republic of Korea.
J Microbiol. 2012 Jun;50(3):547-52. doi: 10.1007/s12275-012-1623-6. Epub 2012 Jun 30.
Strain BS6(T), a Gram-positive non-motile bacterium, was isolated from soil in South Korea and characterized to determine its taxonomic position. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain BS6T belonged to the family Propionibacteriaceae in the class Actinobacteria. Strain BS6(T) showed the highest 16S rRNA gene sequence similarity with Microlunatus soli CC-012602(T) (98.6%) and high sequence similarities with Microlunatus species (94.5-98.6%). Chemotaxonomic data revealed that the predominant fatty acids were anteiso-C(17:0), anteiso-C(15:0), summed feature 8 (C(18:1) ω7c/ω6c), and iso-C(16:0). The cell wall peptidoglycan contained (LL)-diaminopimelic acid, and the major polar lipids were diphosphatidylglycerol, and phosphatidylglycerol. Based on these data, BS6(T) (=KCTC 19858(T) =JCM 17661(T) =CCARM 9244(T) =KEMC 9004-079(T)) should be classified as a type strain of a novel species, for which the name Microlunatus terrae sp. nov. is proposed.
菌株 BS6(T) 是一株革兰氏阳性、非运动性的细菌,从韩国的土壤中分离得到,并对其进行了分类学特征的研究。基于 16S rRNA 基因序列的系统发育分析表明,BS6(T)菌株属于放线菌纲的丙酸杆菌科。BS6(T)菌株与 Microlunatus soli CC-012602(T)(98.6%)的 16S rRNA 基因序列相似度最高,与 Microlunatus 属的其他种(94.5-98.6%)也具有较高的相似度。化学分类学数据表明,该菌的主要脂肪酸为 anteiso-C(17:0)、anteiso-C(15:0)、summed feature 8 (C(18:1) ω7c/ω6c)和 iso-C(16:0)。细胞壁肽聚糖含有 (LL)-二氨基庚二酸,主要极性脂为双磷脂酰甘油和磷脂酰甘油。基于这些数据,BS6(T)(=KCTC 19858(T)=JCM 17661(T)=CCARM 9244(T)=KEMC 9004-079(T))应被分类为一个新种的模式菌株,现提议将其命名为 Terrae 短杆菌 Microlunatus terrae sp. nov.。