Zhou Jue, Qu Fan
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China.
Afr J Tradit Complement Altern Med. 2011;8(5 Suppl):33-45. doi: 10.4314/ajtcam.v8i5S.13. Epub 2011 Jul 3.
The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.
综述了菘蓝生物碱和吲哚硫苷（GLs）的提取、分离及分析方法。采用了不同的分析方法，如高压液相色谱（HPLC）、电喷雾电离质谱联用液相色谱（LC/ESI/MS）、电喷雾电离飞行时间质谱（ESI-TOF-MS）和核磁共振（NMR）来验证和鉴定这些成分。这些方法可对菘蓝中的生物碱和GLs进行快速分离、鉴定和定量测定。通过将不同的检测器与HPLC联用，如PDA、ELSD、ESI-和APCI-MS，在正离子和负离子模式下，可采用至少两种独立的检测模式检测复杂化合物。通过ESI-TOF-MS数据的第二步可推导分子式。但对于某些成分，紫外和质谱无法提供足够的结构鉴定信息。在峰纯化后，通过半制备HPLC进行的NMR可作为一种补充方法。
