采用蛋白质组学技术发现和鉴定小鼠肾母细胞瘤的血清标志物。

Discovery and identification of serum biomarkers of Wilms' tumor in mice using proteomics technology.

机构信息

Department of Urologic Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.

出版信息

Chin Med J (Engl). 2012 May;125(10):1727-32.

Abstract

BACKGROUND

Wilms' tumor (nephroblastoma) is a cancer of the kidneys that occurs typically in children and rarely in adults. Early diagnosis is very important for the treatment and prognosis of the disease. The aim of our study was to discover and identify potential non-invasive and convenient biomarkers for the diagnosis of Wilms' tumor.

METHODS

Nude mice were used to construct a Wilms' tumor model by injecting nephroblastoma cells into their bilateral abdomen. We collected 94 serum samples from mice consisting of 45 samples with Wilms' tumor and 49 controls. The serum proteomic profiles of the samples were analyzed via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. The candidate biomarkers were purified by high-performance liquid chromatography, identified by liquid chromatography-mass spectrometry, and validated using ProteinChip immunoassays.

RESULTS

We finally retrieved two differential proteins (m/z 4509.2; 6207.9), which were identified as apolipoprotein A-II and polyubiquitin, respectively. The expression of apolipoprotein A-II was higher in the Wilms' tumor group than in the control group (P < 0.01). By contrast, the expression of polyubiquitin was lower in the Wilms' tumor group than in the control group.

CONCLUSION

Apolipoprotein A-II and polyubiquitin may be used as potential biomarkers for nephroblastoma in children, and the analysis of apolipoprotein A-II may help diagnose and treat Wilms' tumor.

摘要

背景

肾母细胞瘤（Wilms 瘤）是一种发生于儿童、罕见于成人的肾脏癌症。早期诊断对疾病的治疗和预后非常重要。我们的研究目的是发现和鉴定潜在的、用于 Wilms 瘤诊断的非侵入性和方便的生物标志物。

方法

我们通过将肾母细胞瘤细胞注射到裸鼠双侧腹部构建 Wilms 瘤模型。共收集了 94 份来自小鼠的血清样本，其中 45 份样本患有 Wilms 瘤，49 份为对照。通过表面增强激光解吸/电离飞行时间质谱分析样本的血清蛋白质组图谱。通过高效液相色谱法对候选生物标志物进行纯化，通过液相色谱-质谱法进行鉴定，并使用 ProteinChip 免疫分析进行验证。