Sun Xiaoli, Zhang Kaibin, Ji Xiuhong, Wang Yanwen, Jeffrey Zidichouski, Tong Yan, Gao Huimin, Zhang Junzeng, Wang Zhimin
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2012 May;37(9):1319-23.
The present study was conducted to develop new inhibitors of pancreatic lipase and alpha-glucosidase from Chinese dietary herbs. Sixty-three dietary herbs from 39 taxonomic families were selected and extracted with aqueous ethanol or water. The extracts were then tested with in vitro enzyme assays for their ability to inhibit pancreatic lipase and alpha-glucosidase activities. Orlistat and acarbose were used as two positive controls. The extracts of Nelumbo nucifera, Curcuma longa, Piper longum and Morus alba showed strong pancreatic lipase inhibitory effects with IC50 at (28.00 +/- 5.51), (5.24 +/- 0.51), (14.76 +/- 2.58), (4.78 +/- 0.58), (3.41 +/- 0.67) mg x L(-1), respectively. These extracts also showed potent alpha-glucosidase inhibitory activities with IC50 at (1.98 +/- 0.13), (0. 18 + 0.007), (0.71 +/- 0.08), (0.077 +/- 0.005), (0.089 +/- 0.006) g x L(-1), respectively. The results provide useful information for developing new drugs or natural health products for hyperlipidemia and hypoglycemia from Chinese dietary herbs.
本研究旨在从中国药食同源植物中开发新型胰脂肪酶和α-葡萄糖苷酶抑制剂。选取了39个分类科的63种药食同源植物，用乙醇水溶液或水进行提取。然后通过体外酶测定法检测提取物抑制胰脂肪酶和α-葡萄糖苷酶活性的能力。奥利司他和阿卡波糖用作两个阳性对照。莲、姜黄、荜茇和桑的提取物对胰脂肪酶显示出较强的抑制作用，IC50分别为(28.00±5.51)、(5.24±0.51)、(14.76±2.58)、(4.78±0.58)、(3.41±0.67)mg·L-1。这些提取物对α-葡萄糖苷酶也显示出较强的抑制活性，IC50分别为(1.98±0.13)、(0.18±0.007)、(0.71±0.08)、(0.077±0.005)、(0.089±0.006)g·L-1。该结果为从中国药食同源植物中开发治疗高脂血症和低血糖症的新药或天然保健品提供了有用信息。
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