Suppr超能文献

纳米二氧化硅负载离子液体添加剂对人碳酸酐酶 II 热可逆性的影响。

Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II.

机构信息

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

出版信息

Int J Biol Macromol. 2012 Dec;51(5):933-8. doi: 10.1016/j.ijbiomac.2012.07.023. Epub 2012 Jul 22.

DOI:10.1016/j.ijbiomac.2012.07.023
PMID:22829053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3677219/
Abstract

Silica nanoparticle supported imidazolium ionic liquid [SNImIL] was synthesized and utilized as a biocompatible additive for studying the thermal reversibility of human carbonic anhydrase II (HCA II). For this purpose, we prepared additive by modification of nanoparticles through the grafting of ionic liquids on the surface of nanoparticles (SNImIL). The SNImIL were fully characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and thermo gravimetric analysis. The characterization of HCA II was investigated by various techniques including UV-vis and ANS fluorescence spectrophotometry, differential scanning calorimetry, and docking study. SNImIL induced disaggregation, enhanced protein stability and increased thermal reversibility of HCA II by up to 42% at pH 7.75.

摘要

合成了二氧化硅纳米颗粒负载的咪唑鎓离子液体[SNImIL]，并将其用作研究人碳酸酐酶 II（HCA II）热可逆性的生物相容性添加剂。为此，我们通过将离子液体接枝到纳米颗粒表面来对纳米颗粒进行修饰，制备了添加剂（SNImIL）。通过傅里叶变换红外光谱、扫描电子显微镜和热重分析对 SNImIL 进行了全面表征。通过包括紫外-可见和 ANS 荧光分光光度法、差示扫描量热法和对接研究在内的各种技术研究了 HCA II 的特性。在 pH 7.75 时，SNImIL 诱导 HCA II 解聚，增强了蛋白质稳定性，并使 HCA II 的热可逆性提高了 42%。

相似文献

1
Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II.
Int J Biol Macromol. 2012 Dec;51(5):933-8. doi: 10.1016/j.ijbiomac.2012.07.023. Epub 2012 Jul 22.
2
Enhancement of thermal reversibility and stability of human carbonic anhydrase II by mesoporous nanoparticles.
Int J Biol Macromol. 2015 Apr;75:67-72. doi: 10.1016/j.ijbiomac.2015.01.019. Epub 2015 Jan 17.
3
Modified β-casein restores thermal reversibility of human carbonic anhydrase II: the salt bridge mechanism.
Biotechnol Appl Biochem. 2013 May-Jun;60(3):298-304. doi: 10.1002/bab.1081. Epub 2013 Apr 28.
4
Imidazolium-based ionic liquid functionalized mesoporous silica nanoparticles as a promising nano-carrier: response surface strategy to investigate and optimize loading and release process for Lapatinib delivery.
Pharm Dev Technol. 2020 Nov;25(9):1150-1161. doi: 10.1080/10837450.2020.1803909. Epub 2020 Aug 9.
5
A spectroscopic study on the absorption of carbonic anhydrase onto the nanoporous silica nanoparticle.
Int J Biol Macromol. 2017 Jun;99:739-745. doi: 10.1016/j.ijbiomac.2017.03.030. Epub 2017 Mar 8.
6
Fluorescent Silica Nanoparticles with Multivalent Inhibitory Effects towards Carbonic Anhydrases.
Chemistry. 2015 Jul 13;21(29):10306-9. doi: 10.1002/chem.201501037. Epub 2015 May 12.
7
Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.
FEBS J. 2015 Apr;282(8):1445-57. doi: 10.1111/febs.13232. Epub 2015 Mar 23.
8
Spectroscopic study on the interaction of celecoxib with human carbonic anhydrase II: thermodynamic characterization of the binding process.
J Photochem Photobiol B. 2009 Dec 2;97(3):161-8. doi: 10.1016/j.jphotobiol.2009.09.005. Epub 2009 Sep 19.
9
Nanocomposites of ionic liquids confined in mesoporous silica gels: preparation, characterization and performance.
Phys Chem Chem Phys. 2010 Feb 28;12(8):1971-81. doi: 10.1039/b920556j. Epub 2010 Jan 25.
10
Silica-supported sulfonic acid-functionalized ionic liquid coated with [bmim][PF6] as a scavenger for the synthesis of amides.
Mol Divers. 2011 Feb;15(1):109-13. doi: 10.1007/s11030-010-9261-6. Epub 2010 Oct 6.

引用本文的文献

1
The Effect of Nanoparticles on the Structure and Enzymatic Activity of Human Carbonic Anhydrase I and II.
Molecules. 2020 Sep 25;25(19):4405. doi: 10.3390/molecules25194405.
2
D, L-Sulforaphane Loaded Fe3O4@ Gold Core Shell Nanoparticles: A Potential Sulforaphane Delivery System.
PLoS One. 2016 Mar 16;11(3):e0151344. doi: 10.1371/journal.pone.0151344. eCollection 2016.

本文引用的文献

1
Chaperone-like activity of nanoparticles of hydrophobized poly(vinyl alcohol).
Soft Matter. 2007 May 23;3(6):718-724. doi: 10.1039/b618779j.
2
Impact of the 237th residue on the folding of human carbonic anhydrase II.
Int J Mol Sci. 2011;12(5):2797-807. doi: 10.3390/ijms12052797. Epub 2011 Apr 28.
3
Effect of ionic liquids on the structure, stability and activity of two related α-amylases.
Int J Biol Macromol. 2011 Jan 1;48(1):93-7. doi: 10.1016/j.ijbiomac.2010.10.001. Epub 2010 Oct 12.
4
Ionic liquids as refolding additives: variation of the anion.
J Biotechnol. 2010 Oct 1;150(1):64-72. doi: 10.1016/j.jbiotec.2010.07.003. Epub 2010 Jul 12.
5
Caseins: utilizing molecular chaperone properties to control protein aggregation in foods.
J Agric Food Chem. 2010 Jan 27;58(2):685-93. doi: 10.1021/jf903072g.
6
Ionogels as drug delivery system: one-step sol-gel synthesis using imidazolium ibuprofenate ionic liquid.
Chem Commun (Camb). 2010 Jan 14;46(2):228-30. doi: 10.1039/b913879j. Epub 2009 Nov 16.
7
Spectroscopic study on the interaction of celecoxib with human carbonic anhydrase II: thermodynamic characterization of the binding process.
J Photochem Photobiol B. 2009 Dec 2;97(3):161-8. doi: 10.1016/j.jphotobiol.2009.09.005. Epub 2009 Sep 19.
8
Dual behavior of sodium dodecyl sulfate as enhancer or suppressor of insulin aggregation and chaperone-like activity of camel alphaS(1)-casein.
Int J Biol Macromol. 2009 Dec 1;45(5):511-7. doi: 10.1016/j.ijbiomac.2009.08.008. Epub 2009 Aug 31.
9
Synthesis and biological applications of imidazolium-based polymerized ionic liquid as a gene delivery vector.
Chem Biol Drug Des. 2009 Sep;74(3):282-8. doi: 10.1111/j.1747-0285.2009.00858.x.
10
Effect of additives on protein aggregation.
Curr Pharm Biotechnol. 2009 Jun;10(4):400-7. doi: 10.2174/138920109788488941.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验