阴性对照肽与生物活性肽对皮肤癌细胞和正常细胞的细胞毒性研究：一项对比研究。
Investigation of cytotoxicity of negative control peptides versus bioactive peptides on skin cancer and normal cells: a comparative study.
机构信息
Biotechnology & Environmental Biology, School of Applied Sciences, Science Engineering & Health College, RMIT University, Bundoora, Victoria, 3083, Australia.
出版信息
Future Med Chem. 2012 Aug;4(12):1553-65. doi: 10.4155/fmc.12.98.
BACKGROUND
Resonant recognition model-myxoma virus (RRM-MV), a bioactive peptide analogue for myxoma virus MV-T5 protein, was computationally designed by the RRM. In this study, the anticancer effects of RRM-MV were assessed in vitro against four negative control peptides on human skin cancer and normal cells.
RESULTS & DISCUSSION: The effects of RRM-MV versus negative control peptides on cells were evaluated by quantitative and qualitative assays. The RRM-MV treatment was able to induce cell death in cancer cells without triggering similar effects on normal cells. However, the negative control peptides produced no toxic effects on skin cancer and normal cells. No effects on human erythrocytes were detected when treated with all peptides.
CONCLUSION
It is suggested that the RRM can be applied to design therapeutic anticancer peptides.
背景
谐振识别模型-兔粘液瘤病毒（RRM-MV）是兔粘液瘤病毒 MV-T5 蛋白的生物活性肽类似物，由 RRM 计算设计。在这项研究中，评估了 RRM-MV 对体外四种阴性对照肽对人皮肤癌和正常细胞的抗癌作用。
结果与讨论
通过定量和定性测定评估了 RRM-MV 与阴性对照肽对细胞的影响。RRM-MV 处理能够诱导癌细胞死亡，而对正常细胞没有类似的作用。然而，阴性对照肽对皮肤癌和正常细胞没有产生毒性作用。用所有肽处理时，对人红细胞均无影响。
结论
建议 RRM 可用于设计治疗性抗癌肽。
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