2011 年 GeFI 协作研究。一致性研究、能力验证和意大利人群中新的 ENFSI/EDNAP 基因座 D1S1656、D2S441、D10S1248、D12S391、D22S1045 的数据。
The 2011 GeFI collaborative exercise. Concordance study, proficiency testing and Italian population data on the new ENFSI/EDNAP loci D1S1656, D2S441, D10S1248, D12S391, D22S1045.
机构信息
Dipartimento di Sanità Pubblica, Neuroscienze, Medicina Sperimentale e Forense, Sezione di Medicina Legale e Scienze Forensi, Università di Pavia, Italy.
出版信息
Forensic Sci Int Genet. 2013 Jan;7(1):e15-8. doi: 10.1016/j.fsigen.2012.08.001. Epub 2012 Aug 20.
The 2011 collaborative exercise of the ISFG Italian Working Group GeFI was aimed at validating the five ENFSI/EDNAP miniSTR loci D1S1656, D2S441, D10S1248, D12S391 and D22S1045. The protocol required to type at least 50 multilocus profiles from locally resident individuals and two blind bloodstains in duplicate (i.e., using at least two different commercial kits), and to send the electropherograms to the Organizing Committee. Nineteen laboratories distributed across Italy participated, collecting a total of 960 samples. Full concordance was found for the five new miniSTRs as observed from the comparison of 13,150 alleles. The inspection of the electropherograms allowed the identification of a very limited number of mistypings in the miniSTR genotypes thus contributing to the establishment of an high quality Italian database of frequencies.
2011 年，ISFG 意大利工作组 GeFI 的协作性研究旨在验证 ENFSI/EDNAP 五个微卫星位点 D1S1656、D2S441、D10S1248、D12S391 和 D22S1045。该方案要求从当地居民个体中至少对 50 个多位点图谱进行分型，并对两份盲血斑进行重复分型（即使用至少两种不同的商业试剂盒），然后将电泳图谱发送给组织委员会。分布在意大利各地的 19 个实验室参与了该研究，共收集了 960 个样本。通过对 13150 个等位基因的比较，发现这五个新的微卫星位点完全一致。通过对电泳图谱的检查，发现微卫星基因型中存在非常有限数量的误判，这有助于建立一个高质量的意大利频率数据库。
Zotero 插件