Qu Peng, Li Jin-Tao, Wang Li-Dong, Zeng Yi, Chu Xiu-Sheng
The College of Life Science, Shandong Normal University, Jinan 250014, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2012 Feb;26(1):34-6.
Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions.
Extracted the total DNA from the novel fresh tissue and Paraffin-embedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio); Compared the statistical result from the different specimens; analyzed the result between different region.
HPV infection rate of fresh tissue is 82.6% with HPV16 (34.8%) and HPV18 (34.8%), and paraffin-embedded specimens is 78.2% with HPV16 (30.4%) and HPV18 (17.4%).
The results provides the first evidence that there wasn't noticeable difference between HPV infection rate of the two specimens. So broader specimen source could be used for HPV testing.
对比分析食管鳞状细胞癌新鲜组织与石蜡包埋标本中HPV感染率，并与其他地区检测结果进行比较。
从新鲜组织及石蜡包埋标本中提取总DNA；用通用引物通过PCR检测DNA，并用基于核酸扩增的人乳头瘤病毒基因分型检测试剂盒（Hybribio）检测HPV类型；比较不同标本的统计结果；分析不同地区的结果。
新鲜组织HPV感染率为82.6%，其中HPV16占34.8%，HPV18占34.8%；石蜡包埋标本HPV感染率为78.2%，其中HPV16占30.4%，HPV18占17.4%。
结果首次证明两种标本的HPV感染率无显著差异。因此可使用更广泛的标本来源进行HPV检测。
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