Albrecht U, Seulberger H, Schwarz H, Risau W
Max-Planck-Institut für Psychiatrie, Abteilung Neurochemie, Martinsried, F.R.G.
Brain Res. 1990 Dec 3;535(1):49-61. doi: 10.1016/0006-8993(90)91823-y.
The HT7 protein defined by a monoclonal antibody is a specific marker for chick brain endothelial cells (EMBO J., 5 (1986) 3179-3183). In this study, we have investigated the expression of this protein in the brain circumventricular organs which lack a blood-brain barrier. Using immunohistochemical techniques we found that the protein was absent from the vascular system of the pituitary, median eminence, subfornical organ, pineal gland, the organum vasculosum lamina terminalis and the layer of sinusoid blood vessels of the area postrema. In some regions of the median eminence and, more strikingly, in the pineal gland, parenchymal cells expressed the HT7 antigen. Immunoblots of proteins from brain, pituitary, pineal gland and retina showed that the antigen is very abundant in the retina. Lower amounts were present in brain and pineal gland. The glucose transporter was found to be an independent reliable marker for blood-brain barrier endothelium. In the chick brain the distribution of the biochemically distinct proteins was very similar. Using a postembedding technique we have ultrastructurally localized the HT7 protein specifically in blood-brain barrier endothelial cells. Thus, the expression of the HT7 protein and glucose transporter correlated with blood-brain barrier function.
一种由单克隆抗体所界定的HT7蛋白，是鸡脑内皮细胞的一种特异性标志物（《欧洲分子生物学组织杂志》，5 (1986) 3179 - 3183）。在本研究中，我们调查了该蛋白在缺乏血脑屏障的脑室周器官中的表达情况。运用免疫组织化学技术，我们发现垂体、正中隆起、穹窿下器官、松果体、终板血管器以及最后区的窦状血管层的血管系统中不存在该蛋白。在正中隆起的一些区域，更显著的是在松果体中，实质细胞表达HT7抗原。来自脑、垂体、松果体和视网膜的蛋白质免疫印迹显示，该抗原在视网膜中非常丰富。在脑和松果体中的含量较低。葡萄糖转运蛋白被发现是血脑屏障内皮细胞的一种独立可靠的标志物。在鸡脑中，生化特性不同的蛋白质的分布非常相似。运用包埋后技术，我们在超微结构水平上把HT7蛋白特异性定位在血脑屏障内皮细胞中。因此，HT7蛋白和葡萄糖转运蛋白的表达与血脑屏障功能相关。
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