豆科热带树种扭叶合欢九个转移简单重复序列（SSR）标记的特征分析

Characterization of nine transferred SSR markers in the tropical tree species Enterolobium contortisiliquum (Fabaceae).

作者信息

Moreira P A, Sousa S A S, Oliveira F A, Araújo N H, Fernandes G W, Oliveira D A

机构信息

Laboratório de Ecologia Evolutiva e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

出版信息

Genet Mol Res. 2012 Oct 11;11(4):3729-34. doi: 10.4238/2012.August.17.11.

DOI:10.4238/2012.August.17.11

PMID:22930434

Abstract

Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of polymorphism was assessed in 8 trees sampled from each of 5 populations from central Brazil. All loci were polymorphic and the mean number of alleles for all loci was 6. In addition, all pairs of SSR markers were in linkage equilibrium. For most loci, the observed heterozygosity was higher than the expected heterozygosity under Hardy-Weinberg equilibrium, with fixation indices not significantly different from zero. The combined probability of paternity exclusion was high and the probability of identity was very low. We conclude that these SSR markers developed for E. cyclocarpum are applicable for genetic studies of E. contortisiliquum.

摘要

分子标记的转移在保护遗传学研究中被广泛应用。我们研究了为象耳豆（Enterolobium cyclocarpum）开发的简单序列重复（SSR）标记向扭果象耳豆（E. contortisiliquum）的可转移性，扭果象耳豆是一种广泛分布于干旱森林的热带树木。在扭果象耳豆中扩增了一组9个评估的SSR标记，并在从巴西中部5个种群中各抽取的8棵树中评估了多态性程度。所有位点均为多态性，所有位点的等位基因平均数为6。此外，所有SSR标记对均处于连锁平衡。对于大多数位点，观察到的杂合度高于哈迪-温伯格平衡下的预期杂合度，固定指数与零无显著差异。排除父系的联合概率很高，同一性概率非常低。我们得出结论，这些为象耳豆开发的SSR标记适用于扭果象耳豆的遗传学研究。