三种不同白细胞滤除滤器过滤后血小板活化及膜糖蛋白Ib和IIb-IIIa表达的比较

Comparison of platelet activation and membrane glycoprotein Ib and IIb-IIIa expression after filtration through three different leukocyte removal filters.

作者信息

Bertolini F, Rebulla P, Porretti L, Sirchia G

机构信息

Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, Milano, Italia.

出版信息

Vox Sang. 1990;59(4):201-4. doi: 10.1111/j.1423-0410.1990.tb00236.x.

DOI:10.1111/j.1423-0410.1990.tb00236.x

PMID:2293458

Abstract

We evaluated three filters used for leukocyte removal from platelet concentrates: Imugard IG 500, Pall PL100 and Sepacell PL-10A. Filter performance, platelet activation and expression of membrane glycoproteins Ib and IIb-IIIa were evaluated. Imugard, Pall and Sepacell showed median postfiltration in vitro platelet recoveries of 88, 84 and 80%, and total residual leukocyte counts of 16.1, 7.5 and 0.6 x 10(6)/pool of 8 platelet concentrates, respectively. Mean platelet volume was reduced after filtration with all filters. Postfiltration values of glycoproteins Ib and IIb-IIIa, and of activation markers GMP 140 and gp 53 were not significantly different from prefiltration values. Filtration through Imugard, Pall and Sepacell did not induce significant platelet activation or modifications of platelet membrane glycoproteins Ib and IIb-IIIa.

摘要

我们评估了三种用于从血小板浓缩物中去除白细胞的滤器

Imugard IG 500、颇尔PL100和Sepacell PL - 10A。对滤器性能、血小板活化以及膜糖蛋白Ib和IIb - IIIa的表达进行了评估。Imugard、颇尔和Sepacell滤器过滤后体外血小板回收率的中位数分别为88%、84%和80%，8单位血小板浓缩物混合样本中总的残留白细胞计数分别为16.1×10⁶、7.5×10⁶和0.6×10⁶。使用所有滤器过滤后平均血小板体积均减小。糖蛋白Ib和IIb - IIIa以及活化标志物GMP 140和gp 53的过滤后值与过滤前值无显著差异。通过Imugard、颇尔和Sepacell滤器过滤不会诱导显著的血小板活化或血小板膜糖蛋白Ib和IIb - IIIa的改变。