一些药用植物提取物对诱导大鼠阿尔茨海默病氧化应激状态的影响。
Effect of some medicinal plant extracts on the oxidative stress status in Alzheimer's disease induced in rats.
机构信息
Department of Medical Biochemistry, National Research Center, Cairo University, Cairo, Egypt.
出版信息
Eur Rev Med Pharmacol Sci. 2012 Jul;16 Suppl 3:31-42.
BACKGROUND/AIM: Alzheimer's disease (AD) is a progressive neurodegenerative disorder. Increased oxidative stress has been shown to be a prominent and early feature in AD. Medicinal plants with antioxidant activities have been used traditionally in the treatment of several human diseases. The present study aims to investigate the effect of Salvia triloba and Piper nigrum plant extracts on the oxidative stress status in Alzheimer's disease induced in rats.
MATERIALS AND METHODS
70 male rats were enrolled in this study and were classified into 7 groups (ten each). Group 1: control group, group 2: AD-induced rats by aluminum chloride, and served as positive control; group 3: AD group treated with Rivastigmine in a dose of 0.3 mg/kg b. wt. daily for three months; group 4 & 5: AD group treated with total extract of Salvia triloba in a dose of 750 or 375 mg/kg b. wt. respectively, daily for three months; group 6 & 7: AD group treated with total extract Piper nigrum in a dose of 187.5 or 93.75 mg/kg b. wt. respectively, daily for three months. After three months of treatment animals' sera and brain samples were collected. Malondialdehyde (MDA), nitric oxide (NO) and total antioxidant capacity (TAC) were determined in serum while superoxide dismutase (SOD) in erythrocyte. Brain samples were divided sagitally into two portions, the first portion was separated for determination of acetylcholine (Ach) and acetycholinesterase (AchE). The second portion was used for histopathological investigation.
RESULTS
The results indicated that extracts of Salvia triloba and Piper nigrum as well as Rivastigmine showed significant increase in brain Ach, serum TAC and SOD and significant decreases in brain AchE, MDA and NO in AD-induced rats. Moreover, histological investigation of brain sections showing nearly normal histological structure of hippocampus. Treatment with Salvia triloba in a dose of 750 mg/kg b. wt. was more powerful in protection from Alzheimer's disease than Piper nigrum, as indicated by both biochemical and histopathological findings.
CONCLUSION
This study revealed that the treatment of AD-induced rats with Salvia triloba and Piper nigrum, total plant extracts significantly reduced the oxidative stress status and ameliorates the neurodegeneration characteristic of Alzheimer's diseases in rats. Noteworthy, Salvia triloba extract showed more interest in improvement Alzheimer's disease in rats.
背景/目的：阿尔茨海默病（AD）是一种进行性神经退行性疾病。氧化应激增加已被证明是 AD 的一个突出和早期特征。具有抗氧化活性的药用植物传统上用于治疗多种人类疾病。本研究旨在探讨 Salvia triloba 和 Piper nigrum 植物提取物对铝诱导的 AD 大鼠氧化应激状态的影响。
材料和方法
将 70 只雄性大鼠纳入本研究，并分为 7 组（每组 10 只）。第 1 组：对照组；第 2 组：用氯化铝诱导的 AD 大鼠，作为阳性对照；第 3 组：用 Rivastigmine 以 0.3mg/kg b.wt.的剂量每天治疗 3 个月；第 4 组和第 5 组：用总提取物 Salvia triloba 以 750 或 375mg/kg b.wt.的剂量分别每天治疗 3 个月；第 6 组和第 7 组：用总提取物 Piper nigrum 以 187.5 或 93.75mg/kg b.wt.的剂量分别每天治疗 3 个月。治疗 3 个月后，采集动物血清和脑组织样本。测定血清中丙二醛（MDA）、一氧化氮（NO）和总抗氧化能力（TAC），红细胞中超氧化物歧化酶（SOD）。脑样本沿矢状面分为两部分，第一部分用于测定乙酰胆碱（Ach）和乙酰胆碱酯酶（AchE）。第二部分用于组织病理学检查。
结果
结果表明，Salvia triloba 和 Piper nigrum 提取物以及 Rivastigmine 可显著增加 AD 诱导大鼠的脑 Ach、血清 TAC 和 SOD，并显著降低脑 AchE、MDA 和 NO。此外，脑切片的组织学研究显示海马的组织学结构几乎正常。与 Piper nigrum 相比，Salvia triloba 以 750mg/kg b.wt.的剂量治疗 AD 诱导的大鼠在保护方面更有效，这一点在生化和组织病理学发现中均得到证实。
结论
本研究表明，用 Salvia triloba 和 Piper nigrum 全植物提取物治疗 AD 诱导的大鼠可显著降低氧化应激状态，并改善大鼠阿尔茨海默病的神经退行性特征。值得注意的是，Salvia triloba 提取物在改善大鼠阿尔茨海默病方面表现出更大的兴趣。
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