Yu Jun, Chen Chongda, Wang Jun, Cheng Yu, Wu Qirong, Zhong Yisheng, Shen Xi
Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025;
Exp Ther Med. 2012 Apr;3(4):617-620. doi: 10.3892/etm.2012.457. Epub 2012 Jan 19.
The aim of this study was to investigate the expression of Kir 2.1 and Kir 4.1 channels at an elevated hydrostatic pressure in vitro, and to determine whether adenosine may modulate the mRNA expression of Kir 2.1 and Kir 4.1 channels in retinal Müller cells at an elevated hydrostatic pressure in vitro. Müller cells treated with 1 μM adenosine at 40 mmHg/24 h, and mRNA expression of Kir 2.1 and Kir 4.1 channels were examined using real-time PCR. Müller cells significantly increased the mRNA expression of Kir 2.1 and Kir 4.1 channels at 40 mmHg/24 h. When further treated with 1 μM adenosine at 40 mmHg/24 h, the mRNA expression of the Kir 2.1 channels decreased, while the mRNA expression of the Kir 4.1 channels continued to increase. When the pressure was elevated, Müller cells were still able to take up K(+) and mediate the potassium concentration of the retina. Adenosine upregulated the expression of the Kir 4.1 channels, but weakly affected the expression of the Kir 2.1 channels.
本研究的目的是在体外高静水压条件下研究Kir 2.1和Kir 4.1通道的表达，并确定腺苷是否可在体外高静水压条件下调节视网膜Müller细胞中Kir 2.1和Kir 4.1通道的mRNA表达。用1 μM腺苷处理Müller细胞，压力为40 mmHg/24小时，并用实时PCR检测Kir 2.1和Kir 4.1通道的mRNA表达。在40 mmHg/24小时时，Müller细胞中Kir 2.1和Kir 4.1通道的mRNA表达显著增加。当在40 mmHg/24小时时进一步用1 μM腺苷处理，Kir 2.1通道的mRNA表达下降，而Kir 4.1通道的mRNA表达继续增加。当压力升高时，Müller细胞仍能够摄取K(+)并调节视网膜的钾浓度。腺苷上调了Kir 4.1通道的表达，但对Kir 2.1通道的表达影响较弱。
