Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fitokou Street, GR-38446 Nea Ionia, Volos, Greece.
J Sci Food Agric. 2013 Mar 30;93(5):1184-90. doi: 10.1002/jsfa.5873. Epub 2012 Sep 14.
Fresh fish and seafoods are very perishable products mainly owing to microbial activity of specific spoilage micro-organisms. Application of hurdle technology leads to a variety of processed products with extended shelf life. In this study, sensory evaluation and microbiological analysis were carried out on 17 processed seafood products stored at 4 °C to determine their shelf life and the predominant spoilage micro-organisms.
Shelf life determined by sensory analysis varied from 66 to 180 days depending on the product. The cause of spoilage for most of the products was the development of off-flavours/off-odours, while two products were rejected owing to oil discolouration. Pseudomonads were in most cases below detection limit. H₂S-producing bacteria, Brochothrix thermosphacta and Enterobacteriaceae were below detection limit throughout the experiment. The predominant spoilage micro-organisms were lactic acid bacteria and yeasts. Hygiene indicators such as Staphylococcus spp. and total coliforms were also below detection limit in all samples.
Primarily the initial pH and secondarily the NaCl content determined shelf life duration. Under the applied conditions, lactic acid bacteria and yeasts predominated. The contribution of chemical oxidation and/or autolysis to spoilage and shelf life might be important for most of the products.
新鲜的鱼类和海鲜产品主要由于特定腐败微生物的微生物活动而非常易腐。应用障碍技术可生产出具有延长保质期的各种加工产品。在这项研究中,对 17 种在 4°C 下储存的加工海鲜产品进行了感官评估和微生物分析,以确定其保质期和主要腐败微生物。
根据产品的不同,感官分析确定的保质期从 66 天到 180 天不等。大多数产品变质的原因是产生异味/气味,而有两种产品由于油变色而被拒绝。假单胞菌在大多数情况下低于检测限。在整个实验过程中,产硫化氢细菌、嗜热解糖菌和肠杆菌科均低于检测限。主要的腐败微生物是乳酸菌和酵母菌。在所有样品中,卫生指标如金黄色葡萄球菌和总大肠菌群也低于检测限。
主要是初始 pH 值,其次是 NaCl 含量决定了保质期的长短。在应用的条件下,乳酸菌和酵母菌占优势。对于大多数产品来说,化学氧化和/或自溶对腐败和保质期的贡献可能很重要。